Low Anticoagulant Heparin Disrupts Plasmodium falciparum Rosettes in Fresh Clinical Isolates

The binding of Plasmodium falciparum parasitized erythrocytes to uninfected erythrocytes (rosetting) is associated with severe malaria. The glycosaminoglycan heparan sulfate is an important receptor for rosetting. The related glycosaminoglycan heparin was previously used in treatment of severe malar...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2011-03, Vol.84 (3), p.390-396
Main Authors: LEITGEB, Anna M, BLOMQVIST, Karin, CHO-NGWA, Fidelis, SAMJE, Moses, NDE, Peter, TITANJI, Vincent, WAHLGREN, Mats
Format: Article
Language:English
Subjects:
Adolescent
Animals
Antimalarials - chemistry
Antimalarials - pharmacology
Biological and medical sciences
Blood. Blood coagulation. Reticuloendothelial system
Cameroon - epidemiology
Child
Child, Preschool
Erythrocytes - parasitology
Heparin - analogs & derivatives
Heparin - pharmacology
Heparin, Low-Molecular-Weight - pharmacology
Humans
Infant
Infectious diseases
Malaria, Falciparum - blood
Malaria, Falciparum - epidemiology
Malaria, Falciparum - parasitology
Medical sciences
Pharmacology. Drug treatments
Plasmodium falciparum
Plasmodium falciparum - drug effects
Plasmodium falciparum - immunology
Rosette Formation
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Summary:The binding of Plasmodium falciparum parasitized erythrocytes to uninfected erythrocytes (rosetting) is associated with severe malaria. The glycosaminoglycan heparan sulfate is an important receptor for rosetting. The related glycosaminoglycan heparin was previously used in treatment of severe malaria, although abandoned because of the occurrence of severe bleedings. Instead, low anticoagulant heparin (LAH) has been suggested for treatment. LAH has successfully been evaluated in safety studies and found to disrupt rosettes and cytoadherence in vitro and in vivo in animal models, but the effect of LAH on fresh parasite isolates has not been studied. Herein, we report that two different LAHs (DFX232 and Sevuparin) disrupt rosettes in the majority of fresh isolates from Cameroonian children with malaria. The rosette disruption effect was more pronounced in isolates from complicated cases than from mild cases. The data support LAH as adjunct therapy in severe malaria.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2011.10-0256