In situ quantification of HER2–protein tyrosine kinase 6 (PTK6) protein–protein complexes in paraffin sections from breast cancer tissues

Background: Protein tyrosine kinase 6 (PTK6; breast tumour kinase) is overexpressed in up to 86% of the invasive breast cancers, and its association with the oncoprotein human epidermal growth factor receptor 2 (HER2) was shown in vitro by co-precipitation. Furthermore, expression of PTK6 in tumours...

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Bibliographic Details
Published in:British journal of cancer 2010-08, Vol.103 (5), p.663-667
Main Authors: Aubele, M, Spears, M, Ludyga, N, Braselmann, H, Feuchtinger, A, Taylor, K J, Lindner, K, Auer, G, Stering, K, Höfler, H, Schmitt, M, Bartlett, J M S
Format: Article
Language:English
Subjects:
631/45/475/2290
692/53/2422
692/699/67/1347
Antibodies
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Breast cancer
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cancer Research
Drug Resistance
Environmental health
Epidemiology
Epidermal growth factor
Female
Gynecology. Andrology. Obstetrics
Humans
Kinases
Mammary gland diseases
Medical research
Medical sciences
Molecular Medicine
Neoplasm Proteins - metabolism
Oncology
Paraffin Embedding
Pathology
Protein Binding
Protein-Tyrosine Kinases - metabolism
Proteins
Receptor, ErbB-2 - metabolism
Short Communication
Signal transduction
Tumors
Yeast
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Summary:Background: Protein tyrosine kinase 6 (PTK6; breast tumour kinase) is overexpressed in up to 86% of the invasive breast cancers, and its association with the oncoprotein human epidermal growth factor receptor 2 (HER2) was shown in vitro by co-precipitation. Furthermore, expression of PTK6 in tumours is linked with the expression of HER2. Method and results: In this study, we used the proximity ligation assay (PLA) technique on formalin-fixed paraffin sections from eighty invasive breast carcinoma tissue specimens to locate PTK6–HER2 protein–protein complexes. Proximity ligation assay signals from protein complexes were assessed quantitatively, and expression levels showed a statistically significant association with tumour size ( P =0.015) and course of the cancer disease ( P =0.012). Conclusion: Protein tyrosine kinase 6 forms protein complexes with HER2 in primary breast cancer tissues, which can be visualised by use of the PLA technique. Human epidermal growth factor receptor 2–PTK6 complexes are of prognostic relevance.
ISSN:0007-0920
1532-1827
DOI:10.1038/sj.bjc.6605836