Spatial and temporal regulation of gene expression in the mammalian growth plate

Abstract Growth plates are spatially polarized and structured into three histologically and functionally distinct layers—the resting zone (RZ), proliferative zone (PZ), and hypertrophic zone (HZ). With age, growth plates undergo functional and structural senescent changes including declines of growt...

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Bibliographic Details
Published in:Bone (New York, N.Y.) N.Y.), 2010-05, Vol.46 (5), p.1380-1390
Main Authors: Lui, Julian C.K, Andrade, Anenisia C, Forcinito, Patricia, Hegde, Anita, Chen, WeiPing, Baron, Jeffrey, Nilsson, Ola
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cellular Senescence - genetics
Cellular Senescence - physiology
Chondrocytes - metabolism
Computational Biology
Extracellular Matrix Proteins - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - genetics
Gene Expression Regulation - physiology
Glycoproteins - genetics
Growth Differentiation Factor 10 - genetics
Growth Plate - cytology
Growth Plate - metabolism
In Situ Hybridization
Medicin och hälsovetenskap
Microarray Analysis
Microdissection
Orthopedics
Polymerase Chain Reaction
Rats
Rats, Sprague-Dawley
Transcription Factors - genetics
Transcription Factors - physiology
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Abstract Growth plates are spatially polarized and structured into three histologically and functionally distinct layers—the resting zone (RZ), proliferative zone (PZ), and hypertrophic zone (HZ). With age, growth plates undergo functional and structural senescent changes including declines of growth rate, proliferation rate, growth plate height and cell number. To explore the mechanisms responsible for spatially-associated differentiation and temporally-associated senescence of growth plate in an unbiased manner, we used microdissection to collect individual growth plate zones from proximal tibiae of 1-week rats and the PZ and early hypertrophic zones of growth plates from 3-, 6-, 9-, and 12-week rats and analyzed gene expression using microarray. We then used bioinformatic approaches to identify significant changes in biological functions, molecular pathways, transcription factors and also to identify specific gene products that can be used as molecular markers for individual zones or for temporal development.
ISSN:8756-3282
1873-2763
1873-2763
DOI:10.1016/j.bone.2010.01.373