Induction of HIV-1-specific cellular and humoral immune responses following immunization with HIV-DNA adjuvanted with activated apoptotic lymphocytes

Abstract Delivery of DNA encoding foreign antigens into mammalian cells can induce adaptive immune responses. There are currently many DNA-based vaccines in clinical trials against infectious diseases and cancer but there is a lack of adjuvants for improvement of responses to DNA-based vaccines. Her...

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Published in:Vaccine 2010-02, Vol.28 (9), p.2080-2087
Main Authors: Brave, Andreas, Johansson, Ulrika, Hallengärd, David, Heidari, Shirin, Gullberg, Hanna, Wahren, Britta, Hinkula, Jorma, Spetz, Anna-Lena
Format: Article
Language:English
Subjects:
Adjuvant
Adjuvants, Immunologic - pharmacology
AIDS Vaccines - genetics
AIDS Vaccines - immunology
Allergy and Immunology
Animals
Apoptosis
Applied microbiology
B-Lymphocytes - immunology
Biological and medical sciences
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Granulocyte-Macrophage Colony-Stimulating Factor - immunology
HIV Antibodies - blood
HIV Antibodies - immunology
HIV Infections - immunology
HIV Infections - prevention & control
HIV-1
HIV-1 - immunology
Human immunodeficiency virus
Humans
Immune system
Immunity, Cellular
Immunity, Humoral
Immunization
Infectious diseases
Lymphocyte Activation
Lymphocytes
Medical research
MEDICIN
Medicin och hälsovetenskap
MEDICINE
Mice
Mice, Inbred C57BL
Microbiology
Miscellaneous
Plasmids
Recombinant Proteins
T-Lymphocytes - immunology
Transfection
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, DNA - genetics
Vaccines, DNA - immunology
Virology
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cited_by cdi_FETCH-LOGICAL-c634t-c53a798ed88a96dc9e2e8e0d03f54ddcb6ee04f69fcfabf191941ec6bc8f51493
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container_issue 9
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container_title Vaccine
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creator Brave, Andreas
Johansson, Ulrika
Hallengärd, David
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Hinkula, Jorma
Spetz, Anna-Lena
description Abstract Delivery of DNA encoding foreign antigens into mammalian cells can induce adaptive immune responses. There are currently many DNA-based vaccines in clinical trials against infectious diseases and cancer but there is a lack of adjuvants for improvement of responses to DNA-based vaccines. Here, we show augmented systemic and mucosa-associated B cell responses after immunization with a cocktail of seven different plasmids (3 env , 2 gag , 1 rev , 1 RT ) combined with mitogen activated apoptotic syngeneic lymphocytes in mice. In addition we show that apoptotic cells can function as adjuvant for induction of cellular immune responses in a magnitude comparable to the cytokine adjuvant GM-CSF in mice. These data suggest that activated apoptotic lymphocytes can act independent as adjuvants to improve antigen-specific DNA vaccines.
doi_str_mv 10.1016/j.vaccine.2009.12.040
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subjects Adjuvant
Adjuvants, Immunologic - pharmacology
AIDS Vaccines - genetics
AIDS Vaccines - immunology
Allergy and Immunology
Animals
Apoptosis
Applied microbiology
B-Lymphocytes - immunology
Biological and medical sciences
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Granulocyte-Macrophage Colony-Stimulating Factor - immunology
HIV Antibodies - blood
HIV Antibodies - immunology
HIV Infections - immunology
HIV Infections - prevention & control
HIV-1
HIV-1 - immunology
Human immunodeficiency virus
Humans
Immune system
Immunity, Cellular
Immunity, Humoral
Immunization
Infectious diseases
Lymphocyte Activation
Lymphocytes
Medical research
MEDICIN
Medicin och hälsovetenskap
MEDICINE
Mice
Mice, Inbred C57BL
Microbiology
Miscellaneous
Plasmids
Recombinant Proteins
T-Lymphocytes - immunology
Transfection
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, DNA - genetics
Vaccines, DNA - immunology
Virology
title Induction of HIV-1-specific cellular and humoral immune responses following immunization with HIV-DNA adjuvanted with activated apoptotic lymphocytes
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