Room-Temperature Infrared Spectroscopy Combined with Mass Spectrometry Distinguishes Gas-Phase Protein Isomers

Differential IR spectra of gas‐phase proteins at room temperature can be obtained by a combination of IR multiphoton dissociation (IRMPD) and FT ion cyclotron resonance mass spectrometry. This new approach yields unique spectra for all‐L and singly D‐substituted isomers of the protein Trp‐cage (see...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2009-01, Vol.48 (44), p.8340-8342
Main Authors: Fung, Y.M. Eva, Besson, Thierry, Lemaire, Joël, Maitre, Philippe, Zubarev, Roman A
Format: Article
Language:English
Subjects:
Chemical Physics
Chemical Sciences
Gases - chemistry
IR spectroscopy
Isomerism
Mass Spectrometry
Medicin och hälsovetenskap
or physical chemistry
Physics
Protein Isoforms - chemistry
protein structures
proteins
Proteins - chemistry
Spectrophotometry, Infrared
Temperature
Theoretical and
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Summary:Differential IR spectra of gas‐phase proteins at room temperature can be obtained by a combination of IR multiphoton dissociation (IRMPD) and FT ion cyclotron resonance mass spectrometry. This new approach yields unique spectra for all‐L and singly D‐substituted isomers of the protein Trp‐cage (see depicted differential spectrum) and thus avoids possible unfolding of proteins during IR irradiation that can affect conventional IRMPD measurements.
ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.200901516