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RNA binding protein CsrA controls cyclic di-GMP metabolism by directly regulating the expression of GGDEF proteins

The carbon storage regulator CsrA is an RNA binding protein that controls carbon metabolism, biofilm formation and motility in various eubacteria. Nevertheless, in Escherichia coli only five target mRNAs have been shown to be directly regulated by CsrA at the post-transcriptional level. Here we iden...

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Published in:Molecular microbiology 2008-10, Vol.70 (1), p.236-257
Main Authors: Jonas, Kristina, Edwards, Adrianne N, Simm, Roger, Romeo, Tony, Römling, Ute, Melefors, Öjar
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description The carbon storage regulator CsrA is an RNA binding protein that controls carbon metabolism, biofilm formation and motility in various eubacteria. Nevertheless, in Escherichia coli only five target mRNAs have been shown to be directly regulated by CsrA at the post-transcriptional level. Here we identified two new direct targets for CsrA, ycdT and ydeH, both of which encode proteins with GGDEF domains. A csrA mutation caused mRNA levels of ycdT and ydeH to increase more than 10-fold. RNA mobility shift assays confirmed the direct and specific binding of CsrA to the mRNA leaders of ydeH and ycdT. Overexpression of ycdT and ydeH resulted in a more than 20-fold increase in the cellular concentration of the second messenger cyclic di-GMP (c-di-GMP), implying that both proteins possess diguanylate cyclase activity. Phenotypic characterization revealed that both proteins are involved in the regulation of motility in a c-di-GMP-dependent manner. CsrA was also found to regulate the expression of five additional GGDEF/EAL proteins and a csrA mutation led to modestly increased cellular levels of c-di-GMP. All together, these data demonstrate a global role for CsrA in the regulation of c-di-GMP metabolism by regulating the expression of GGDEF proteins at the post-transcriptional level.
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subjects Bacterial proteins
Binding sites
Biological and medical sciences
Carbon
Cyclic GMP - analogs & derivatives
Cyclic GMP - metabolism
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Eubacteria
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genome, Bacterial
Genotype
Medicin och hälsovetenskap
Metabolism
Microbiology
Microscopy, Atomic Force
Mutagenesis, Site-Directed
Mutation
Oligonucleotide Array Sequence Analysis
Phenotype
Plasmids
Protein Binding
Repressor Proteins - genetics
Repressor Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA Processing, Post-Transcriptional
RNA, Bacterial - metabolism
RNA, Messenger - metabolism
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
title RNA binding protein CsrA controls cyclic di-GMP metabolism by directly regulating the expression of GGDEF proteins
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