Characterization of neuroprogenitor cells expressing the PDGF β-receptor within the subventricular zone of postnatal mice

• Published in: Molecular and cellular neuroscience 2008-03, Vol.37 (3), p.507-518
• Main Authors: Ishii, Yoko, Matsumoto, Yoshiki, Watanabe, Rie, Elmi, Muna, Fujimori, Toshihiko, Nissen, Johan, Cao, Yihai, Nabeshima, Yo-Ichi, Sasahara, Masakiyo, Funa, Keiko
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; Animals, Newborn; Benzamides; Bromodeoxyuridine; Bromodeoxyuridine - metabolism; Cell and Molecular Biology; Cell Differentiation; Cell Differentiation - drug effects; Cell Differentiation - genetics; Cell Movement; Cell Movement - drug effects; Cell Movement - physiology; Cell- och molekylärbiologi; Cells; Cells, Cultured; Cultured; cytology; drug effects; Enzyme Inhibitors; Enzyme Inhibitors - pharmacology; FGF; Gene Expression; Gene Expression - drug effects; Gene Expression - physiology; genetics; growth & development; Imatinib Mesylate; Inbred C57BL; Intermediate Filament Proteins; Intermediate Filament Proteins - genetics; Intermediate Filament Proteins - metabolism; Ki-67 Antigen; Ki-67 Antigen - metabolism; Lateral Ventricles; Lateral Ventricles - cytology; Lateral Ventricles - growth & development; Medicin och hälsovetenskap; metabolism; methods; Mice; Mice, Inbred C57BL; Mice, Transgenic; Nerve Tissue Proteins; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Nestin; Neural stem cells; Neurons; Neurons - physiology; Neurosphere; Newborn; PDGF-receptor; pharmacology; physiology; Piperazines; Piperazines - pharmacology; Platelet-Derived Growth Factor beta; Pyrimidines; Pyrimidines - pharmacology; Receptor; Receptor, Platelet-Derived Growth Factor beta - genetics; Receptor, Platelet-Derived Growth Factor beta - metabolism; Stem Cells; Stem Cells - metabolism; Transfection; Transfection - methods; Transgenic
• ISSN: 1044-7431; 1095-9327; 1095-9327
• DOI: 10.1016/j.mcn.2007.11.006

We report a considerable number of cells in the ventricular and the subventricular zones (SVZ) of newborn mice to stain positive for the PDGF β-receptor (PDGFRB). Many of them also stained for nestin and/or GFAP but less frequently for the neuroblast marker doublecortin and for the mitotic marker Ki-67. The SVZ of mice with nestin-Cre conditional deletion of PDGFRB expressed the receptor only on blood vessels and was devoid of any morphological abnormality. PDGFRB −/− neurospheres showed a higher rate of apoptosis without any significant decrease in proliferation. They demonstrated reduced capacities of migration and neuronal differentiation in response to not only PDGF-BB but also bFGF. Furthermore, the PDGFR kinase inhibitor STI571 blocked the effects of bFGF in control neurosphere cultures. bFGF increased the activity of the PDGFRB promoter as well as the expression and phosphorylation of PDGFRB. These results suggest the presence of the signaling convergence between PDGF and FGF. PDGFRB is needed for survival, and the effects of bFGF in migration and neural differentiation of the cells may be potentiated by induction of PDGFRB.