Toward a Confocal Subcellular Atlas of the Human Proteome

Information on protein localization on the subcellular level is important to map and characterize the proteome and to better understand cellular functions of proteins. Here we report on a pilot study of 466 proteins in three human cell lines aimed to allow large scale confocal microscopy analysis us...

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Bibliographic Details
Published in:Molecular & cellular proteomics 2008-03, Vol.7 (3), p.499-508
Main Authors: Barbe, Laurent, Lundberg, Emma, Oksvold, Per, Stenius, Anna, Lewin, Erland, Björling, Erik, Asplund, Anna, Pontén, Fredrik, Brismar, Hjalmar, Uhlén, Mathias, Andersson-Svahn, Helene
Format: Article
Language:English
Subjects:
Antibodies, Neoplasm
Bioengineering
Bioteknik
Cell Compartmentation
Cell Line, Tumor
Humans
Internet
MEDICIN
Medicin och hälsovetenskap
MEDICINE
Microscopy, Confocal
Protein Transport
Proteome - metabolism
Subcellular Fractions
TECHNOLOGY
TEKNIKVETENSKAP
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Summary:Information on protein localization on the subcellular level is important to map and characterize the proteome and to better understand cellular functions of proteins. Here we report on a pilot study of 466 proteins in three human cell lines aimed to allow large scale confocal microscopy analysis using protein-specific antibodies. Approximately 3000 high resolution images were generated, and more than 80% of the analyzed proteins could be classified in one or multiple subcellular compartment(s). The localizations of the proteins showed, in many cases, good agreement with the Gene Ontology localization prediction model. This is the first large scale antibody-based study to localize proteins into subcellular compartments using antibodies and confocal microscopy. The results suggest that this approach might be a valuable tool in conjunction with predictive models for protein localization.
ISSN:1535-9476
1535-9484
1535-9484
DOI:10.1074/mcp.M700325-MCP200