Increased Expression of Specific Thioredoxin Family Proteins; A Pilot Immunohistochemical Study on Human Hepatocellular Carcinoma

Hepatocellular Carcinoma (HCC) is one of the most frequent cancers worldwide, however, prognosis remains poor following its discovery. We investigate the Thioredoxin superfamily of proteins as diagnostic markers for HCC. Furthermore, we delineate possible roles of the endoplasmic reticulum member of...

Full description

Saved in:
Bibliographic Details
Published in:International journal of immunopathology and pharmacology 2007-01, Vol.20 (1), p.17-24
Main Authors: Cunnea, P., Fernandes, A.P., Capitanio, A., Eken, S., Spyrou, G., Björnstedt, M.
Format: Article
Language:English
Subjects:
Carcinoma, Hepatocellular - metabolism
Cell Line
Cell Line, Tumor
DNA, Complementary - biosynthesis
DNA, Complementary - genetics
Doxorubicin - therapeutic use
Drug Resistance, Neoplasm
Endoplasmic Reticulum - physiology
Glyceraldehyde-3-Phosphate Dehydrogenases - genetics
Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism
HSP40 Heat-Shock Proteins
Humans
Immunohistochemistry
Liver Neoplasms - metabolism
Medicin och hälsovetenskap
Molecular Chaperones - immunology
Paraffin Embedding
Pilot Projects
Reverse Transcriptase Polymerase Chain Reaction
RNA - biosynthesis
RNA - isolation & purification
RNA, Small Interfering - pharmacology
Stress, Physiological - chemically induced
Stress, Physiological - metabolism
Thioredoxins - biosynthesis
Tunicamycin - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hepatocellular Carcinoma (HCC) is one of the most frequent cancers worldwide, however, prognosis remains poor following its discovery. We investigate the Thioredoxin superfamily of proteins as diagnostic markers for HCC. Furthermore, we delineate possible roles of the endoplasmic reticulum member of the superfamily, ERdj5, in carcinogenesis. Using antibodies against Thioredoxin 1, Thioredoxin Reductase 1 and ERdj5, we performed immunohistochemistry on paraffin embedded liver biopsy sections from HCC patients. All three redox proteins exhibited elevated expression levels in tumor tissue compared to internal control, with ERdj5 showing a remarkable 3-fold increase. In vitro cell viability experiments using Hepatocellular Carcinoma HuH7 cells treated with ERdj5 small interfering RNA showed that ERdj5 knockdown cells exhibited less resistance to Doxorubicin (chemotherapy drug), but more resistance to Tunicamycin (Endoplasmic Stress inducer), compared to control cells. In conclusion, we introduce members of the Thioredoxin superfamily as possible immunohistochemical markers in the diagnostics of hepatocellular carcinoma and indicate a potential defensive role for ERdj5 in chemotherapeutic drug resistance.
ISSN:0394-6320
2058-7384
DOI:10.1177/039463200702000103