Imaging Technique for the Screening of Protein−Protein Interactions Using Scattered Light under Surface Plasmon Resonance Conditions

We propose a novel technique to detect protein−protein interactions in microarray format. The technique involves measuring scattered light under surface plasmon resonance (SPR) conditions. We have shown that the maximum scattering angle correlates with the traditionally employed reflection minimum....

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2007-02, Vol.79 (4), p.1349-1355
Main Authors: Savchenko, Andrej, Kashuba, Elena, Kashuba, Vladimir, Snopok, Boris
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Biochemistry
Blood Proteins - analysis
Chemistry
Equipment Design
Equipment Failure Analysis
Exact sciences and technology
General, instrumentation
Immunoglobulin G - analysis
Light
Protein Array Analysis - instrumentation
Protein Array Analysis - methods
Protein Binding
Protein Interaction Mapping - instrumentation
Protein Interaction Mapping - methods
Proteins
Rabbits
Scattering
Scattering, Radiation
Scientific imaging
Sensitivity and Specificity
Spectrometric and optical methods
Staphylococcal Protein A - analysis
Staphylococcus aureus
Surface Plasmon Resonance - instrumentation
Surface Plasmon Resonance - methods
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Summary:We propose a novel technique to detect protein−protein interactions in microarray format. The technique involves measuring scattered light under surface plasmon resonance (SPR) conditions. We have shown that the maximum scattering angle correlates with the traditionally employed reflection minimum. Panoramic scanning of scattered light under SPR conditions has all the functional advantages of the SPR technique. In addition, the proposed technique simplifies device design, increases the dynamic range of analysis, and integrates data with those from surface-plasmon field-enhanced fluorescence spectroscopy. We demonstrate the technique by showing direct protein−protein interaction between protein A and either rabbit antibodies or human serum.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac061456n