Differential Pattern of Human Blood Neutrophil Activation After Stimulation With Organic Dust in Vitro and in Vivo

Objective: To study activation of blood neutrophils in subjects after in vitro and in vivo stimulation. Methods: Blood from healthy volunteers was collected before and after their exposure to organic dust in a pig farm. Surface markers of neutrophil activation were analyzed by flow cytometry. Result...

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Bibliographic Details
Published in:Journal of occupational and environmental medicine 2007-02, Vol.49 (2), p.131-138
Main Authors: Jonsson, Eva Wikström, Palmberg, Lena
Format: Article
Language:English
Subjects:
Adult
Agriculture
Air
Air Pollutants, Occupational - blood
Animals
Biological and medical sciences
Blood
Dust
Environmental pollutants toxicology
Female
Flow Cytometry
Humans
In Vitro Techniques
Inhalation Exposure
Leukocytosis - classification
Leukocytosis - etiology
Male
Medical sciences
Middle Aged
Neutrophil Activation - physiology
Occupational hazards
Occupational health
ORIGINAL ARTICLES
Peroxidase - blood
Swine
Swine houses
Toxicology
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Summary:Objective: To study activation of blood neutrophils in subjects after in vitro and in vivo stimulation. Methods: Blood from healthy volunteers was collected before and after their exposure to organic dust in a pig farm. Surface markers of neutrophil activation were analyzed by flow cytometry. Results: In vivo, there was a diurnal variation in basal levels of CD11b. Swine dust exposure increased the number of blood neutrophils and the levels of myeloperoxidase without effects on CD11b or Cys-X-Cys receptor 1 (CXCR-1) expression. However, a positive correlation between changes of myeloperoxidase and CD11b expression was observed. In vitro, N-formyl-methionyl-leucyl-phenylalanine (fMLP), lipopolysaccharide (LPS), leukotriene LT)B₄, LTD₄, and swine dust stimulated the expression of CD11b. fMLP, LPS, and LTB₄ diminished the expression of CXCR-1. Conclusions: Exposure to swine dust in vitro activated neutrophils and inhalation of swine dust caused neutrophilia.
ISSN:1076-2752
1536-5948
DOI:10.1097/JOM.0b013e31802f3c4a