Antimicrobial Peptide LL‐37 Internalized by Immature Human Dendritic Cells Alters their Phenotype

The human cathelicidin LL‐37 has been shown to be involved in the barrier function of the innate immunity, being released from specific cells upon challenge and exerting immunomodulatory effects. We here demonstrate that LL‐37 affects immature dendritic cells, derived from human peripheral blood mon...

Full description

Saved in:
Bibliographic Details
Published in:Scandinavian journal of immunology 2006-06, Vol.63 (6), p.410-419
Main Authors: Bandholtz, L., Ekman, G. Jacobsson, Vilhelmsson, M., Buentke, E., Agerberth, B., Scheynius, A., Gudmundsson, G. H.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antimicrobial Cationic Peptides - metabolism
Antimicrobial Cationic Peptides - physiology
Cell Differentiation - immunology
Cells, Cultured
Cytokines - biosynthesis
Dendritic Cells - cytology
Dendritic Cells - immunology
Dendritic Cells - metabolism
Humans
Immunity, Cellular
Immunophenotyping
Intracellular Fluid - immunology
Intracellular Fluid - metabolism
Medicin och hälsovetenskap
Molecular Sequence Data
Monocytes - cytology
Monocytes - immunology
Monocytes - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The human cathelicidin LL‐37 has been shown to be involved in the barrier function of the innate immunity, being released from specific cells upon challenge and exerting immunomodulatory effects. We here demonstrate that LL‐37 affects immature dendritic cells, derived from human peripheral blood monocytes (MDDC). LL‐37 is internalized by MDDC with subsequent localization primarily in the cytoplasmic compartment. However, LL‐37 could also be detected in the nuclei of MDDC, suggesting that LL‐37 may be transported into the nucleus. The uptake of LL‐37 is dose, time and energy dependent, indicating that the observed internalization process involves an endocytic pathway. Incubation of immature MDDC with LL‐37 caused phenotypic changes, characterized by an increased expression of the antigen‐presenting molecule HLA‐DR, and the costimulatory molecule CD86. Taken together, these findings suggest that LL‐37 released upon triggering of the innate immunity, may affect cellular adaptive immunity through an interaction with immature dendritic cells.
ISSN:0300-9475
1365-3083
DOI:10.1111/j.1365-3083.2006.001752.x