Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: The involvement of ICAM-1

• Published in: Biochemical and biophysical research communications 2005-12, Vol.338 (4), p.1825-1833
• Main Authors: Domeij, Helena, Modéer, Thomas, Quezada, Hernán Concha, Yucel-Lindberg, Tülay
• Format: Article
• Language: English
• Subjects: Adolescent; Antibodies - pharmacology; Cells, Cultured; Child; Child, Preschool; Co-cultures; Coculture Techniques; Fibroblasts - metabolism; Gingiva - cytology; Gingival fibroblasts; Humans; ICAM-1; Imidazoles - pharmacology; Intercellular Adhesion Molecule-1 - biosynthesis; Intercellular Adhesion Molecule-1 - immunology; Intercellular Adhesion Molecule-1 - physiology; MAP Kinase Signaling System - physiology; Matrix Metalloproteinase 1 - biosynthesis; MMP-1; Monocytes; Monocytes - metabolism; p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors; Pyridines - pharmacology; TIMP-1; Tissue Inhibitor of Metalloproteinase-1 - biosynthesis
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2005.10.137

Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. The aim of this study was to investigate the cellular expression of MMP-1 and its inhibitor, tissue inhibitor of metalloproteinase-1 (TIMP-1), in gingival fibroblasts co-cultured with monocytes and the possible mediating role of intercellular adhesion molecule-1 (ICAM-1). In co-cultures, the expression of MMP-1 and TIMP-1 increased in fibroblasts, but not in monocytes, although the number of MMP-1 + and TIMP-1 + adhered monocytes increased. Moreover, ICAM-1 expression in both fibroblasts and adhered monocytes increased. In the presence of an anti-ICAM-1 antibody, the expression of MMP-1 in fibroblasts decreased whereas the number of TIMP-1 + adhered monocytes increased. The p38 MAPK inhibitor SB203580 reduced MMP-1 expression in fibroblasts, as well as ICAM-1 expression in both fibroblasts and adhered monocytes. The results suggest that co-culture with monocytes enhances cellular expression of MMP-1 and TIMP-1 in gingival fibroblasts, and that the increased MMP-1 expression, in contrast to TIMP-1, is partly mediated by the adhesion molecule ICAM-1 and the p38 MAPK signal pathway.