Signaling molecules and cytokine production in T cells of patients with B-cell chronic lymphocytic leukemia (B-CLL): comparison of indolent and progressive disease

T-cell dysfunction in B-CLL patients might be attributed to altered expression of components of the TCR/CD3 complex and associated intracellular tyrosine kinases. Four-color flow cytometry was applied to the expression of these molecules as well as the T-cell regulatory cytokines (IFN-gamma and IL-4...

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Bibliographic Details
Published in:Medical oncology (Northwood, London, England) London, England), 2005-01, Vol.22 (3), p.291-302
Main Authors: Kiaii, Shahryar, Choudhury, Aniruddha, Mozaffari, Fariba, Kimby, Eva, Osterborg, Anders, Mellstedt, Håkan
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
Aged
Aged, 80 and over
Antigens, CD - analysis
Cytokines - analysis
Cytokines - biosynthesis
Disease Progression
Female
Flow Cytometry
Humans
Leukemia
Leukemia, Lymphocytic, Chronic, B-Cell - immunology
Leukemia, Lymphocytic, Chronic, B-Cell - pathology
Male
Medicin och hälsovetenskap
Middle Aged
Oncology
Prognosis
T-Lymphocytes - immunology
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Summary:T-cell dysfunction in B-CLL patients might be attributed to altered expression of components of the TCR/CD3 complex and associated intracellular tyrosine kinases. Four-color flow cytometry was applied to the expression of these molecules as well as the T-cell regulatory cytokines (IFN-gamma and IL-4) in B-CLL patients with indolent and progressive disease. Intracellular levels [mean fluorescent intensity (MFI)] of IFN-gamma and IL-4 in both CD4 and CD8 T cells of both patient groups were significantly higher than in healthy donors. Absolute number of IL-4 producing CD4 T cells in patients with indolent was significantly higher than in healthy donors. The expression level (MFI) of the CD3-zeta chain was higher in patients than in normal donors as well as ZAP-70 in patients with indolent disease as compared to healthy donors and progressive patients. No significant difference was noted in the expression of p56lck, p59fyn, and PI3-kinase between healthy donors and patients or between the patient subgroups. The results indicate multiple T-cell abnormalities especially in indolent-stage B-CLL suggesting a state of chronic and aberrant activation. This information might be of significance when studying the immunobiology of B-CLL as well as developing new therapeutic approaches.
ISSN:1357-0560
1357-0560
1559-131X
DOI:10.1385/MO:22:3:291