Functional replacement of Drosophila Btk29A with human Btk in male genital development and survival

Drosophila type 2 Btk29A reveals the highest homology to Btk among mammalian Tec kinases. In Btk29A ficP mutant males, the apodeme holding the penis split into two pieces. Human Btk rescued this phenotype in 39% of Btk29A ficP males, while the Drosophila transgenes did so in 90–100% of mutants. The...

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Bibliographic Details
Published in:FEBS letters 2005-08, Vol.579 (19), p.4131-4137
Main Authors: Hamada, Noriko, Bäckesjö, Carl-Magnus, Smith, C.I. Edvard, Yamamoto, Daisuke
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Bruton's tyrosine kinase
Btk
Drosophila
Drosophila - genetics
Evolutionary conservation
Genital development
Genitalia, Male - embryology
Humans
Longevity
Male
Medicin och hälsovetenskap
Mutation
Phenotype
Protein-Tyrosine Kinases - genetics
Protein-Tyrosine Kinases - physiology
Transgenes
X-linked agammaglobulinemia
XLA
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Summary:Drosophila type 2 Btk29A reveals the highest homology to Btk among mammalian Tec kinases. In Btk29A ficP mutant males, the apodeme holding the penis split into two pieces. Human Btk rescued this phenotype in 39% of Btk29A ficP males, while the Drosophila transgenes did so in 90–100% of mutants. The Btk29A ficP mutation reduced adult longevity to 11% that of wild-type. This effect was counteracted by Drosophila type 2, yielding 76% of the wild-type lifespan. Human Btk extended the lifespan of Btk29A ficP mutants only to 20% that of wild-type. Thus human Btk can partially replace Drosophila Btk29A + in male genital development and survival.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2005.06.042