Acquired sulphonamide resistance genes in faecal Escherichia coli from healthy children in Bolivia and Peru

Antimicrobial resistance and sulphonamide resistance determinants were studied in 20 co-trimoxazole resistant Escherichia coli in faecal samples from healthy children in Bolivia and Peru. Methods used were disc diffusion susceptibility tests, PCR, sequence analysis and plasmid conjugation assays. Al...

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Bibliographic Details
Published in:International journal of antimicrobial agents 2005-04, Vol.25 (4), p.308-312
Main Authors: Infante, Berónica, Grape, Malin, Larsson, Mattias, Kristiansson, Charlotte, Pallecchi, Lucia, Rossolini, Gian Maria, Kronvall, Göran
Format: Article
Language:English
Subjects:
Anti-Infective Agents - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological and medical sciences
Bolivia
Carrier Proteins - genetics
Carrier Proteins - metabolism
Child
Child, Preschool
Co-trimoxazole
Conjugation, Genetic
Dihydropteroate Synthase - genetics
Dihydropteroate Synthase - metabolism
Drug Resistance, Bacterial - genetics
E. coli
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - isolation & purification
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Feces - microbiology
Humans
Infant
Integron
Integrons
Medical sciences
Medicin och hälsovetenskap
Microbial Sensitivity Tests
Peru
Pharmacology. Drug treatments
sul
Sulfonamides - pharmacology
Sulphonamide resistance
Trimethoprim, Sulfamethoxazole Drug Combination - pharmacology
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Summary:Antimicrobial resistance and sulphonamide resistance determinants were studied in 20 co-trimoxazole resistant Escherichia coli in faecal samples from healthy children in Bolivia and Peru. Methods used were disc diffusion susceptibility tests, PCR, sequence analysis and plasmid conjugation assays. All isolates but one were resistant to at least two different classes of antimicrobials; 19 isolates also carried at least one sul-gene. The most frequent gene was sul2 followed by sul1 and sul3, which was detected in one isolate. This is the first observation of sul3 on the American continent. In conclusion, the high prevalence of sul-genes in this material of faecal commensal E. coli isolates points to a potential role of the faecal flora in the emergence and spread of antimicrobial resistance.
ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2004.12.004