In vitro effect of cyclic adenosine 3', 5'-monophosphate (cAMP) on early human ovarian follicles

• Published in: Journal of assisted reproduction and genetics 2004-08, Vol.21 (8), p.301-306
• Main Authors: Zhang, Pu, Louhio, Henna, Tuuri, Timo, Sjöberg, Jari, Hreinsson, Julius, Telfer, Evelyn E, Hovatta, Outi
• Format: Article
• Language: English
• Subjects: 8-Bromo Cyclic Adenosine Monophosphate - pharmacology; Adenosine; Apoptosis; Biopsy; Female; Follicles; Gynecology; Humans; In vitro fertilization; Laparoscopy; Ligands; Medicin och hälsovetenskap; Obstetrics; Ovarian Follicle - cytology; Ovarian Follicle - drug effects; Ovarian Follicle - growth & development; Ovaries; Tissue Culture Techniques - methods
• ISSN: 1058-0468; 1573-7330
• DOI: 10.1023/b:jarg.0000043704.10845.87

To test the effect of cyclic adenosine 3', 5'-monophosphate (cAMP) on early human ovarian follicles during prolonged culture period. Donated ovarian biopsies from 16 women undergoing gynecological laparoscopy were cut into slices and cultured in parallel for 1, 2, or 3 weeks in the presence and the absence of 0.5 mM 8-bromo-cAMP. The developmental stages, sizes, and viability of the follicles were recorded from histological sections of all samples. On day 14, cortical slices cultured with 8-bromo-cAMP showed a significantly higher proportion of secondary follicles (50.0% vs. 20.0%) and a lower proportion of primordial follicles (9.7% vs. 26.7%) when compared with those cultured without 8-bromo-cAMP. On day 21, the proportion of viable follicles in cortical slices with 8-bromo-cAMP treatment was significantly higher than that without 8-bromo-cAMP treatment (79.6% vs. 55.2%). CyclicAMP promoted folliculogenesis and follicle survival during 14-21 days' culture of human ovarian cortical slices.