Biophysical properties of regions flanking the bHLH-Zip motif in the p22 Max protein

The Max protein is the central dimerization partner in the Myc-Max-Mad network of transcriptional regulators, and a founding structural member of the family of basic-helix–loop–helix (bHLH)—leucine zipper (Zip) proteins. Biologically important regions flanking its bHLH-Zip motif have been disordered...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-10, Vol.323 (3), p.750-759
Main Authors: Pursglove, Sharon E., Fladvad, Malin, Bellanda, Massimo, Moshref, Ahmad, Henriksson, Marie, Carey, Jannette, Sunnerhagen, Maria
Format: Article
Language:English
Subjects:
3' Flanking Region - genetics
Amino Acid Motifs
Amino Acid Sequence
Amino Acid Substitution
Bacteriophage P22 - metabolism
Basic-Leucine Zipper Transcription Factors
Binding
Binding Sites
Chymotrypsin
Circular dichroism
Denaturation
DNA, Viral - chemistry
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - genetics
Electrophoretic Mobility Shift Assay - methods
EMSA
Limited proteolysis
Medicin och hälsovetenskap
Molecular Sequence Data
Mutagenesis, Site-Directed
NMR
Protein Binding
Protein Conformation
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Structure-Activity Relationship
TECHNOLOGY
TEKNIKVETENSKAP
Transcription Factors - chemistry
Transcription Factors - genetics
Transcriptional regulator
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Summary:The Max protein is the central dimerization partner in the Myc-Max-Mad network of transcriptional regulators, and a founding structural member of the family of basic-helix–loop–helix (bHLH)—leucine zipper (Zip) proteins. Biologically important regions flanking its bHLH-Zip motif have been disordered or absent in crystal structures. The present study shows that these regions are resistant to proteolysis in both the presence and absence of DNA, and that Max dimers containing both flanking regions have significantly higher helix content as measured by circular dichroism than that predicted from the crystal structures. Nuclear magnetic resonance measurements in the absence of DNA also support the inferred structural order. Deletion of both flanking regions is required to achieve maximal DNA affinity as measured by EMSA. Thus, the previously observed functionalities of these Max regions in DNA binding, phosphorylation, and apoptosis are suggested to be linked to structural properties.
ISSN:0006-291X
1090-2104
1090-2104
DOI:10.1016/j.bbrc.2004.08.166