Arg-vasopressin increases proliferation of human osteoblast-like cells and decreases production of interleukin-6 and macrophage colony-stimulating factor

Patients with arginine-vasopressin (AVP) deficiency have been reported to have a decreased bone mass. The mechanism behind this is not known. In this study, the effects of AVP on primary human osteoblast-like (hOB) cells and SaOS-2 cells were investigated. Cell proliferation was measured by [ 3H]thy...

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Bibliographic Details
Published in:Regulatory peptides 2004-09, Vol.121 (1), p.41-48
Main Authors: Lagumdzija, Alena, Bucht, Elisabet, Stark, André, Hulting, Anna-Lena, Petersson, Maria
Format: Article
Language:English
Subjects:
Alkaloids
Antidiuretic Hormone Receptor Antagonists
Arginine Vasopressin - genetics
Arginine Vasopressin - pharmacology
Benzophenanthridines
Biological and medical sciences
Cell Line
Cell Proliferation - drug effects
Cells, Cultured
Chelerythrine
Chromones - pharmacology
Fundamental and applied biological sciences. Psychology
hOB
Humans
Indomethacin
Indomethacin - pharmacology
Interleukin-6 - biosynthesis
LY 294002
Macrophage Colony-Stimulating Factor - biosynthesis
Medicin och hälsovetenskap
Morpholines - pharmacology
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - metabolism
Phenanthridines - pharmacology
Protein Biosynthesis - drug effects
Receptors, Vasopressin - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
RT-PCR
SaOS-2
V1-antagonist
V1a-receptor
Vertebrates: endocrinology
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Summary:Patients with arginine-vasopressin (AVP) deficiency have been reported to have a decreased bone mass. The mechanism behind this is not known. In this study, the effects of AVP on primary human osteoblast-like (hOB) cells and SaOS-2 cells were investigated. Cell proliferation was measured by [ 3H]thymidine incorporation or a commercially available kit (EZ4U), and protein synthesis by [ 3H]proline incorporation. In addition, the production of interleukin-6 (IL-6) and macrophage colony-stimulating factor (M-CSF) in hOB cells was determined. AVP at 10–100 pmol/l increased cell proliferation in hOB and SaOS-2 cells ( p
ISSN:0167-0115
1873-1686
DOI:10.1016/j.regpep.2004.04.002