Assessment of irritant skin reactions using electrical impedance - a comparison between 2 laboratories

To assess interlaboratory variability in the measurement of skin electrical impedance (IMP), we evaluated irritant reactions to sodium lauryl sulfate (SLS) (2%) and nonanoic acid (NAA) (40%) in 2 laboratories. We studied the patch test responses in 40 healthy male and female volunteers between 20 an...

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Bibliographic Details
Published in:Contact dermatitis 2003-07, Vol.49 (1), p.26-31
Main Authors: Kuzmina, Natalia, Duval, Celine, Johnsson, Stina, Boman, Anders, Lindberg, Magnus, Emtestam, Lennart
Format: Article
Language:English
Subjects:
Adult
Allergic diseases
Analysis of Variance
bioengineering methods
Biological and medical sciences
Calibration
Dermatitis, Irritant - diagnosis
Dermatitis, Irritant - etiology
Electric Impedance
electrical impedance
Fatty Acids - adverse effects
Female
General aspects. Methods
Humans
Immunopathology
irritancy
Irritants - adverse effects
Male
Medical sciences
multicentre study
nonanoic acid
Observer Variation
Patch Tests
Sensitivity and Specificity
Skin allergic diseases. Stinging insect allergies
Sodium Dodecyl Sulfate - adverse effects
sodium lauryl sulfate
Toxicology
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Summary:To assess interlaboratory variability in the measurement of skin electrical impedance (IMP), we evaluated irritant reactions to sodium lauryl sulfate (SLS) (2%) and nonanoic acid (NAA) (40%) in 2 laboratories. We studied the patch test responses in 40 healthy male and female volunteers between 20 and 30 years of age (20 in each laboratory) with an instrument for measuring IMP. 2 other bioengineering methods and visual scoring were also used to facilitate further illumination of any findings. A strict protocol including all details of the measurement procedure was carefully implemented in both laboratories. The skin reactions were evaluated at 23 h and at 3, 7 and 14 days after exposure. Our findings show that both irritants caused distinct dynamic responses detectable with the bioengineering techniques. Interestingly, the IMP baseline values varied between the 2 laboratories. Moreover, at early stages in the development of irritation (day 1), the irritants induced changes in IMP indices in the opposite direction, which accords with the concept of various IMP patterns of contact dermatitis caused by different irritants. Although the absolute values of IMP differed in both laboratories, the dynamic response patterns were the same.
ISSN:0105-1873
1600-0536
DOI:10.1111/j.0105-1873.2003.00139.x