Molecular cloning, structural analysis, and expression of a human IRLB, MYC promoter-binding protein: new DENN domain-containing protein family emerges

IRLB was originally identified as a partial cDNA clone, encoding a 191-aa protein binding the interferon-stimulated response element (ISRE) in the P2 promoter of human MYC. Here, we cloned the full-size IRLB using different bioinformatics tools and an RT-PCR approach. The full-size gene encompasses...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2003-09, Vol.82 (3), p.343-354
Main Authors: Semova, Natalia, Kapanadze, Bagrat, Corcoran, Martin, Kutsenko, Alexei, Baranova, Ancha, Semov, Alexandre
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Bioinformatics
Calmodulin
Cloning
Death Domain Receptor Signaling Adaptor Proteins
DENN
DNA, Complementary
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - genetics
Fibroblasts
Guanine Nucleotide Exchange Factors - genetics
Humans
Medicin och hälsovetenskap
Mice
Molecular Sequence Data
MYC
PPR repeat
Protein Structure, Tertiary
Proto-Oncogene Proteins c-myc - biosynthesis
Proto-Oncogene Proteins c-myc - genetics
RNA, Messenger - metabolism
RT-PCR
Sequence Analysis, DNA
Sequence Analysis, Protein
Serum stimulation
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Summary:IRLB was originally identified as a partial cDNA clone, encoding a 191-aa protein binding the interferon-stimulated response element (ISRE) in the P2 promoter of human MYC. Here, we cloned the full-size IRLB using different bioinformatics tools and an RT-PCR approach. The full-size gene encompasses 131 kb within chromosome 15q22 and consists of 32 exons. IRLB is transcribed as a 6.6-kb mRNA encoding a protein of 1865 aa. IRLB is ubiquitously expressed and its expression is regulated in a growth- and cell cycle-dependent manner. In addition to the ISRE-binding domain IRLB contains a tripartite DENN domain, a nuclear localization signal, two PPRs, and a calmodulin-binding domain. The presence of DENN domains predicts possible interactions of IRLB with GTPases from the Rab family or regulation of growth-induced MAPKs. Strongly homologous proteins were identified in all available vertebrate genomes as well as in Caenorhabditis elegans and Drosophila melanogaster. In human and mouse a family of IRLB proteins exists, consisting of at least three members.
ISSN:0888-7543
1089-8646
DOI:10.1016/S0888-7543(03)00103-4