Rapid Diagnosis of Active Tuberculosis by Detecting Antibodies from Lymphocyte Secretions

In the present study, we investigated the tuberculosis (TB) diagnostic performance of an assay on the basis of detection of TB-specific antibodies from peripheral blood mononuclear cells (PBMCs), to determine whether antibodies in lymphocyte secretions obtained from PBMCs would better reflect active...

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Bibliographic Details
Published in:The Journal of infectious diseases 2003-08, Vol.188 (3), p.364-370
Main Authors: Raqib, Rubhana, Rahman, Jubayer, Kamaluddin, A. K. M., Mostafa Kamal, S. M., Banu, Fauzia A., Ahmed, Shakeel, Rahim, Zeaur, Bardhan, Pradip K., Andersson, Jan, Sack, David A.
Format: Article
Language:English
Subjects:
Acute Disease
Adolescent
Adult
Antibodies
Antibodies, Bacterial - analysis
Antibody-Producing Cells - immunology
Antigens
Bacterial diseases
Biological and medical sciences
Cells, Cultured
Female
Human bacterial diseases
Humans
Immunoassay - methods
Immunoglobulin G - analysis
Infectious diseases
Lymphocytes
Lymphocytes - immunology
Male
Medical sciences
Medicin och hälsovetenskap
Middle Aged
Mycobacterium bovis - immunology
Mycobacterium tuberculosis
Predictive Value of Tests
Prospective Studies
Pulmonary tuberculosis
Secretion
Sensitivity and Specificity
Time Factors
Tuberculin
Tuberculin - immunology
Tuberculosis
Tuberculosis - blood
Tuberculosis - diagnosis
Tuberculosis and atypical mycobacterial infections
Tuberculosis vaccine
Vaccination
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Summary:In the present study, we investigated the tuberculosis (TB) diagnostic performance of an assay on the basis of detection of TB-specific antibodies from peripheral blood mononuclear cells (PBMCs), to determine whether antibodies in lymphocyte secretions obtained from PBMCs would better reflect active disease than antibodies in serum. PBMCs from patients with and without TB cultured in various concentrations for different times were assessed. Immunoglobulin G (IgG) specific for antigen (bacille Calmette-Guérin [BCG] vaccine and purified protein derivative [PPD]) was measured in lymphocyte secretions. Patients with active TB had higher BCG- or PPD-specific IgG antibody responses than patients without TB or healthy subjects (P=.001). This method can be used as a quick diagnostic aid to facilitate rapid detection of TB cases.
ISSN:0022-1899
1537-6613
DOI:10.1086/376511