Measurement of an apoptotic product in the sera of breast cancer patients

During apoptosis, a number of intracellular proteins are cleaved by caspases. The intermediate filament protein cytokeratin 18 (CK18) is cleaved at Asp238 and Asp396. A monoclonal antibody, M30, specifically recognises a fragment of CK18 cleaved at Asp396 (M30-antigen). We used an enzyme-linked immu...

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Bibliographic Details
Published in:European journal of cancer (1990) 2003-04, Vol.39 (6), p.769-774
Main Authors: Ueno, T, Toi, M, Bivén, K, Bando, H, Ogawa, T, Linder, S
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Antibodies, Monoclonal - blood
Antigens, Neoplasm - blood
Apoptosis
Biological and medical sciences
Breast cancer
Breast Neoplasms - blood
Breast Neoplasms - pathology
Caspase
Cytokeratin 18
ELISA
Enzyme-Linked Immunosorbent Assay
Female
Gynecology. Andrology. Obstetrics
Humans
Keratins - blood
Keratins - immunology
M30
Mammary gland diseases
Medical sciences
Medicin och hälsovetenskap
Middle Aged
Receptors, Estrogen - blood
Receptors, Progesterone - blood
Serum
Survival Analysis
Tumors
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Summary:During apoptosis, a number of intracellular proteins are cleaved by caspases. The intermediate filament protein cytokeratin 18 (CK18) is cleaved at Asp238 and Asp396. A monoclonal antibody, M30, specifically recognises a fragment of CK18 cleaved at Asp396 (M30-antigen). We used an enzyme-linked immunosorbent assay (ELISA) to measure M30-antigen levels in the sera of 82 healthy subjects and 201 patients with breast cancer. Patients with primary cancer had higher M30-antigen levels than healthy subjects (P=0.0001). Patients with recurrent cancer showed higher M30-antigen levels than healthy controls and patients with primary cancer (P
ISSN:0959-8049
1879-0852
DOI:10.1016/S0959-8049(02)00865-1