KI-67 AND hTERT Expression Can Aid in the Distinction between Malignant and Benign Pheochromocytoma and Paraganglioma

The clinical and histopathological distinction between benign and malignant pheochromocytomas and paragangliomas is difficult, and reliable diagnostic markers are lacking. Here we have evaluated the prognostic value of human telomerase reverse transcriptase (hTERT) gene expression detected by revers...

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Bibliographic Details
Published in:Modern pathology 2003-03, Vol.16 (3), p.246-255
Main Authors: Elder, E Edström, Xu, D, Höög, A, Enberg, U, Hou, M, Pisa, P, Gruber, A, Larsson, C, Bäckdahl, M
Format: Article
Language:English
Subjects:
Abdominal Neoplasms - metabolism
Abdominal Neoplasms - pathology
Adolescent
Adrenal Gland Neoplasms - metabolism
Adrenal Gland Neoplasms - pathology
Adult
Aged
Antigens, CD
Antigens, Neoplasm
Basigin
Biomarkers, Tumor - metabolism
Diagnosis, Differential
DNA-Binding Proteins
EMMPRIN
Female
Gene Expression
hTERT
Humans
Immunohistochemistry
In Situ Hybridization
Ki-67
Ki-67 Antigen - biosynthesis
Laboratory Medicine
Male
Matrix Metalloproteinase 2 - biosynthesis
Medicine
Medicine & Public Health
Membrane Glycoproteins - biosynthesis
Middle Aged
MMP-2
Neoplasm Invasiveness
original-article
Paraganglioma
Paraganglioma - metabolism
Paraganglioma - pathology
Pathology
Pheochromocytoma
Pheochromocytoma - metabolism
Pheochromocytoma - pathology
Predictive Value of Tests
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Telomerase
Telomerase - biosynthesis
Telomerase - metabolism
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Summary:The clinical and histopathological distinction between benign and malignant pheochromocytomas and paragangliomas is difficult, and reliable diagnostic markers are lacking. Here we have evaluated the prognostic value of human telomerase reverse transcriptase (hTERT) gene expression detected by reverse transcription PCR (RT-PCR); telomerase activity (TA) measured by TRAP (telomeric repeat amplification protocol) assay; immunohistochemical staining for Ki-67/MIB-1; and the mRNA expression of matrix metalloproteinase (MMP)–2 and EMMPRIN (extracellular matrix metalloproteinase inducer) analyzed by in situ hybridization in 32 primary pheochromocytomas or abdominal paragangliomas. hTERT was expressed in 7/11 malignant tumors (defined as presence of metastasis and/or extensive local invasion) as compared with in 2/21 benign tumors. All of the benign tumors showed
ISSN:0893-3952
1530-0285
DOI:10.1097/01.MP.0000056982.07160.E3