Immunization with recombinant Semliki Forest virus induces protection against influenza challenge in mice

The replicon of Semliki Forest virus (SFV) offers the possibility to direct high-level, transient expression of heterologous proteins in vivo. We initiated studies to determine the possibility of employing the SFV expression system for recombinant vaccine purposes. Mice immunized with recombinant SF...

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Bibliographic Details
Published in:Vaccine 1999-02, Vol.17 (5), p.497-507
Main Authors: Berglund, Peter, Fleeton, Marina N., Smerdou, Cristian, Liljeström, Peter
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Hematopoietic Stem Cells - immunology
Immunity, Mucosal
Immunization
Immunoglobulin G - blood
Immunoglobulin G - classification
Immunologic Memory
Influenza A virus
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Microbiology
Orthomyxoviridae Infections - prevention & control
Semliki Forest virus
Semliki forest virus - immunology
T-Lymphocytes, Cytotoxic - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Synthetic - immunology
Viral Vaccines - immunology
Virology
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Summary:The replicon of Semliki Forest virus (SFV) offers the possibility to direct high-level, transient expression of heterologous proteins in vivo. We initiated studies to determine the possibility of employing the SFV expression system for recombinant vaccine purposes. Mice immunized with recombinant SFV encoding Influenza A nucleoprotein (NP) or E. coli LacZ developed long-lasting antigen-specific IgG levels and induction of cytotoxic T-cell (CTL) memory that persisted for over one year. Predominantly type 1 T-helper cells were induced as shown by IgG subclass ELISA. Humoral and cell-mediated immune responses could be induced upon delivery by several administration routes and mucosal immunizations induced secretory IgA in the respiratory tract. Development of immune responses against the vector itself did not inhibit boost responses by subsequent immunizations with recombinant SFV. Immunization of mice with vectors encoding the Influenza A virus antigens nucleoprotein (NP) and hemagglutinin (HA) resulted in immune responses that were protective against challenge infection with Influenza virus.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(98)00224-2