Sensitive magnetic biodetection using magnetic multi-core nanoparticles and RCA coils

We use functionalized iron oxide magnetic multi-core particles of 100nm in size (hydrodynamic particle diameter) and AC susceptometry (ACS) methods to measure the binding reactions between the magnetic nanoparticles (MNPs) and bio-analyte products produced from DNA segments using the rolling circle...

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Bibliographic Details
Published in:Journal of magnetism and magnetic materials 2017, Vol.427, p.14-18
Main Authors: Ahrentorp, Fredrik, Blomgren, Jakob, Jonasson, Christian, Sarwe, Anna, Sepehri, Sobhan, Eriksson, Emil, Kalaboukhov, Alexei, Jesorka, Aldo, Winkler, Dag, Schneiderman, Justin F., Nilsson, Mats, Albert, Jan, de la Torre, Teresa Zardán Gómez, Strømme, Maria, Johansson, Christer
Format: Article
Language:English
Subjects:
AC susceptometry
Amplification
Biodetection
Coiling
Coils
Condensed Matter
Deoxyribonucleic acid
DIAGNOSTICS
Differential
Differential induction
Differential induction coil
DNA
Dynamic response
Engineering Science with specialization in Nanotechnology and Functional Materials
Fysik
Iron oxides
Magnetic fields
Magnetic induction
Magnetic multi-core particles
Magnetic nano-particles
Magnetic nanoparticles
Magnetism
Materials Science
Measurement methods
Multi core
Multidisciplinary
NANOBEADS
Nanomagnetics
Nanoparticles
P221
PARTICLES
Physical Sciences
Physics
RCA coil
RMANY
SENSORS
Teknisk fysik med inriktning mot nanoteknologi och funktionella material
V380
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Description
Summary:We use functionalized iron oxide magnetic multi-core particles of 100nm in size (hydrodynamic particle diameter) and AC susceptometry (ACS) methods to measure the binding reactions between the magnetic nanoparticles (MNPs) and bio-analyte products produced from DNA segments using the rolling circle amplification (RCA) method. We use sensitive induction detection techniques in order to measure the ACS response. The DNA is amplified via RCA to generate RCA coils with a specific size that is dependent on the amplification time. After about 75min of amplification we obtain an average RCA coil diameter of about 1µm. We determine a theoretical limit of detection (LOD) in the range of 11 attomole (corresponding to an analyte concentration of 55 fM for a sample volume of 200µL) from the ACS dynamic response after the MNPs have bound to the RCA coils and the measured ACS readout noise. We also discuss further possible improvements of the LOD. •Biosensing using Brownian relaxation of functionalized magnetic nanoparticles.•Rolling circle amplification and magnetic nanoparticles enables biosensing.•Theoretical limit of detection estimated from the signal noise gives about 55fM.
ISSN:0304-8853
1873-4766
1873-4766
DOI:10.1016/j.jmmm.2016.10.041