Labeling of human C-peptide by conjugation with N-succinimidyl-4-[18F]fluorobenzoate

We have labeled proinsulin connecting peptide (C‐peptide) with fluorine‐18 (t½=109.7 min) in order to perform in vivo biodistribution and pharmacokinetic studies with position emission tomography (PET). This study reports the optimization of the conjugation labeling in the N‐terminal with N‐succinim...

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Bibliographic Details
Published in:Journal of labelled compounds & radiopharmaceuticals 2001-06, Vol.44 (7), p.509-519
Main Authors: Fredriksson, Anna, Johnström, Peter, Stone-Elander, Sharon, Jonasson, Per, Nygren, Per-Åke, Ekberg, Karin, Johansson, Bo-Lennart, Wahren, John
Format: Article
Language:English
Subjects:
Chemistry
connecting peptide
diabetes
Exact sciences and technology
fluorine-18
Organic chemistry
Peptides
PET
Preparations and properties
protein labeling
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Summary:We have labeled proinsulin connecting peptide (C‐peptide) with fluorine‐18 (t½=109.7 min) in order to perform in vivo biodistribution and pharmacokinetic studies with position emission tomography (PET). This study reports the optimization of the conjugation labeling in the N‐terminal with N‐succinimidyl‐4‐[18F]fluorobenzoate ([18F]SFB). In preparative runs N‐4‐[18F]fluorobenzoyl‐C‐peptide ([18F]FB‐C‐peptide) was produced in 8–12% decay‐corrected yields, counted from resolubilized [18F]F−, in less than 5 h. The specific radioactivity of [18F]FB‐C‐peptide, determined using ELISA for one of the preparations, was around 70 GBq/μmol at end of synthesis. Copyright © 2001 John Wiley & Sons, Ltd.
ISSN:0362-4803
1099-1344
DOI:10.1002/jlcr.479