Flow cytometric detection of leukemic blasts in cerebrospinal fluid predicts risk of relapse in childhood acute lymphoblastic leukemia: a Nordic Society of Pediatric Hematology and Oncology study

• Published in: Leukemia 2020-02, Vol.34 (2), p.336-346
• Main Authors: Thastrup, Maria, Marquart, Hanne Vibeke, Levinsen, Mette, Grell, Kathrine, Abrahamsson, Jonas, Albertsen, Birgitte Klug, Frandsen, Thomas Leth, Harila-Saari, Arja, Lähteenmäki, Päivi Maria, Niinimäki, Riitta, Pronk, Cornelis Jan, Ulvmoen, Aina, Vaitkevičienė, Goda, Taskinen, Mervi, Schmiegelow, Kjeld
• Format: Article
• Language: English
• Subjects: 13/1; 13/31; 692/499; 692/699/67/1990/283/2125; Acute lymphoblastic leukemia; Acute lymphocytic leukemia; Adolescent; Blood cell count; Cancer; Cancer Research; Central nervous system; Cerebrospinal fluid; Child; Child, Preschool; Childhood; Children; Cohort Studies; Critical Care Medicine; Diagnosis; Diseases; Female; Flow Cytometry; Hematology; Humans; Incidence; Infant; Intensive; Internal Medicine; Leukemia; Leukocytes; Lymphatic leukemia; Male; Medicin och hälsovetenskap; Medicine; Medicine & Public Health; Minimal residual disease; Neoplasm Recurrence, Local - cerebrospinal fluid; Neoplasm Recurrence, Local - pathology; Oncology; Oncology, Experimental; Pediatrics; Precursor Cell Lymphoblastic Leukemia-Lymphoma - cerebrospinal fluid; Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology; Prospective Studies; Regression analysis; Relapse; Risk; Vincristine
• ISSN: 0887-6924; 1476-5551; 1476-5551
• DOI: 10.1038/s41375-019-0570-1

Central nervous system (CNS) involvement by cytospin is associated with increased risk of relapse in childhood acute lymphoblastic leukemia. We investigated if flow cytometric analysis of cerebrospinal fluid (CSF) at diagnosis improves the prediction of relapse. This prospective cohort study included patients (1.0–17.9 years) treated according to the Nordic Society of Pediatric Hematology and Oncology ALL2008 protocol. CSF flow cytometry samples were obtained at 17 centers, preserved with Transfix®, and analyzed at a central laboratory. One-hundred and seventy-one (25.4%) of 673 patients were positive by flow cytometry (CNS flow+ ). The 4-year cumulative incidence of relapse was higher for patients with cytospin positivity (CNS cyto+ ) (17.1% vs. 7.5%), CNS flow+ (16.5% vs. 5.6%), and cytospin and/or flow positivity (CNS comb+ ) (16.7% vs. 5.1%). In Cox regression analysis stratified by immunophenotype and minimal residual disease day 29 and adjusted by sex, predictors of relapse were age (hazard ratio [HR] 1.1, 95% CI 1.1–1.2, P