Hilbert phase microscopy based on pseudo thermal illumination in the Linnik configuration

Quantitative phase microscopy (QPM) is often based on recording an object-reference interference pattern and its further phase demodulation. We propose pseudo Hilbert phase microscopy (PHPM) where we combine pseudo thermal light source illumination and Hilbert spiral transform (HST) phase demodulati...

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Bibliographic Details
Published in:Optics letters 2022-11, Vol.47 (22), p.5793-5796
Main Authors: Rogalski, Mikołaj, Cywińska, Maria, Ahmad, Azeem, Patorski, Krzysztof, Micó, Vicente, Ahluwalia, Balpreet S, Trusiak, Maciej
Format: Article
Language:English
Subjects:
Fourier transforms
Illumination
Light sources
Microscopy
Phase demodulation
Robustness (mathematics)
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Summary:Quantitative phase microscopy (QPM) is often based on recording an object-reference interference pattern and its further phase demodulation. We propose pseudo Hilbert phase microscopy (PHPM) where we combine pseudo thermal light source illumination and Hilbert spiral transform (HST) phase demodulation to achieve hybrid hardware-software-driven noise robustness and an increase in resolution of single-shot coherent QPM. Those advantageous features stem from physically altering the laser spatial coherence and numerically restoring spectrally overlapped object spatial frequencies. The capabilities of PHPM are demonstrated by analyzing calibrated phase targets and live HeLa cells in comparison with laser illumination and phase demodulation via temporal phase shifting (TPS) and Fourier transform (FT) techniques. The performed studies verified the unique ability of PHPM to combine single-shot imaging, noise minimization, and preservation of phase details.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.471858