Detection of active SARS-CoV-2 in cough aerosols from COVID-19 patients

• Published in: Infectious diseases (London, England) England), 2024, Vol.56 (11), p.1-999
• Main Authors: Rufino de Sousa, Nuno, Margerie, Lucille, Steponaviciute, Laura, Roux, Julie, Kinahan, Matthew W, Olsson, David, Ásgeirsson, Hilmir, Udekwu, Klas I, Rothfuchs, Antonio Gigliotti
• Format: Article
• Language: English
• Subjects: Infectious Medicine; Infektionsmedicin; Medicin och hälsovetenskap
• ISSN: 2374-4235; 2374-4243; 2374-4243
• DOI: 10.1080/23744235.2024.2374307

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an airborne pathogen, but detection of infectious SARS-CoV-2 in air and in particular the introduction of the virus into the environment by different human expiratory manoeuvres is not well studied. The aim of this study was to investigate the presence of SARS-CoV-2 in cough from coronavirus disease of 2019 (COVID-19) in-patients and to study contamination of the virus in the patient's environment. Detection of SARS-CoV-2 in cough was analyzed by PCR, culture and imaging. Detection in cough was compared to presence of the virus in air and on surfaces from patient rooms. Twenty-five patients in 21 rooms were included in the study. SARS-CoV-2 RNA was found in cough aerosols from 16 out of 22 patients that produced voluntary cough. As demonstrated by plaque-forming unit assays, active virus was isolated from 11 of these 16 patients. Using mainly molecular detection, the virus was also found in air, on high-contact surfaces, and no-touch surfaces from the room of the COVID-19 patients. These results show that infectious SARS-CoV-2 circulating in air can originate from patient cough and should be considered against the risk of acquiring COVID-19 through inhalation.