Occurrence of Partial Nuclei in Eggs of the Sand Dollar, Clypeaster japonicus

Females of Clypeaster japonicus bearing eggs with multiple nuclei were occasionally found. DAPI (4′-6-diamidino-2-phenylindole) stained all these nuclei. The summed volume of the two nuclei in binucleate eggs was similar to the nuclear volume in mononucleate eggs from the same batch. On fertilizatio...

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Bibliographic Details
Published in:The Biological bulletin (Lancaster) 1990-10, Vol.179 (2), p.183-185
Main Authors: Yoneda, Mitsuki, Nemoto, Shin-Ichi
Format: Article
Language:English
Subjects:
Aquatic life
Biological and medical sciences
Cell cycle
Clypeaster japonicus
Development and Reproduction
Echinodermata
Echinoderms
Eggs
Female animals
Fertilization
Fundamental and applied biological sciences. Psychology
Germ cells
Invertebrates
Marine
Marine biology
Oocyte donation
Oocytes
Ova
Physiological aspects
Pronucleus
Sand dollars
Sea urchins
Single status
Ultrastructure
Ultrastructure (Biology)
Zygotes
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Description
Summary:Females of Clypeaster japonicus bearing eggs with multiple nuclei were occasionally found. DAPI (4′-6-diamidino-2-phenylindole) stained all these nuclei. The summed volume of the two nuclei in binucleate eggs was similar to the nuclear volume in mononucleate eggs from the same batch. On fertilization, two partial nuclei migrated to the center of the egg with a time-course similar to that taken by a single nucleus; they then participated in forming the zygote nucleus, which subsequently formed a single mitotic spindle. These multiple nuclei thus appear to function as genuine nuclei. Possibly they result from the failure of a single nucleus to form during oogenesis.
ISSN:0006-3185
1939-8697
DOI:10.2307/1541767