Comparison of different reducing sugar assays in relation to their application to glycanase tests

The response of neutral sugar oligomers in different reducing sugar assays was determined. Monomers, dimers and trimers—as well as tetramers—of xylose and galactose were tested on a molar basis for their response in the Nelson-Somogyi assay, the neo-cuproine assay and the ferricyanide assay. The res...

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Bibliographic Details
Published in:Food hydrocolloids 1991, Vol.4 (6), p.481-487
Main Authors: Kormelink, F.J.M., van de Vis, J.W., Leeuwen, M.J.F.Searle-Van, Voragen, A.G.J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Food Chemistry and Microbiology
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Levensmiddelenchemie en -microbiologie
Methods of analysis, processing and quality control, regulation, standards
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Summary:The response of neutral sugar oligomers in different reducing sugar assays was determined. Monomers, dimers and trimers—as well as tetramers—of xylose and galactose were tested on a molar basis for their response in the Nelson-Somogyi assay, the neo-cuproine assay and the ferricyanide assay. The results show increasing response with increasing chain length of the oligomers in the ferricyanide assay and the neo-cuproine assay. The response of the dimers, trimers and tetramers are, however, the same in the Nelson-Somogyi assay. These differences are important when assaying, for instance, endo(1, 4)-β-xylanase and endo(1, 4)-β-galactanase activities or determining their kinetic parameters.
ISSN:0268-005X
1873-7137
DOI:10.1016/S0268-005X(09)80198-7