Unusual structure of the exopolysaccharide of Rhizobium leguminosarum bv. viciae strain 248

The exopolysaccharide from R. leguminosarum bv. viciae strain 248 differs from those of other Rhizobium strains with similar symbiotic behavior. 13C-N.m.r. spectroscopy of fragments generated by partial hydrolysis, together with methylation analysis and 13C-n.m.r. spectroscopy of the enzymically dep...

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Bibliographic Details
Published in:Carbohydrate research 1991-09, Vol.218, p.185-200
Main Authors: Canter Cremers, Hayo C.J., Stevens, Karin, Lugtenberg, Ben J.J., Wijffelman, Carel A., Batley, Michael, Redmond, John W., Breedveld, Michael W., Zevenhuizen, Loek P.T.M.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Carbohydrate Sequence
carbohydrate structures
chemical analysis
Fabaceae - microbiology
Fundamental and applied biological sciences. Psychology
hydrolysis
Laboratorium voor Microbiologie
Magnetic Resonance Spectroscopy
Methylation
Microbiological Laboratory
Microbiologie
Microbiology
Molecular Sequence Data
monosaccharide sequences
monosaccharides
Morphology, structure, chemical composition
Mutagenesis, Insertional
Plants, Medicinal
polysaccharides
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - genetics
Polysaccharides, Bacterial - metabolism
Rhizobium leguminosarum
Rhizobium leguminosarum - chemistry
Rhizobium leguminosarum - genetics
Rhizobium leguminosarum - metabolism
Symbiosis - physiology
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Summary:The exopolysaccharide from R. leguminosarum bv. viciae strain 248 differs from those of other Rhizobium strains with similar symbiotic behavior. 13C-N.m.r. spectroscopy of fragments generated by partial hydrolysis, together with methylation analysis and 13C-n.m.r. spectroscopy of the enzymically depolymerised exopolysaccharide, indicated the following nonasaccharide repeating-unit: ▪ The locations of the acetyl and 3-hydroxybutanoyl substituents in the exopolysaccharide are assigned provisionally. R. leguminosarum bv. viciae strain 248, cured of its Sym plasmid pRL1JI, synthesised an exopolysaccharide in which the sites and degree of substitution were unchanged. A Tn5 mutant, derived from strain 248 and unable to induce nodules, synthesised small amounts of EPS that lacked galactose.
ISSN:0008-6215
1873-426X
DOI:10.1016/0008-6215(91)84097-X