Kinetics of Nitrobacter agilis at extreme substrate, product and salt concentrations

Measurement and description of the effects of extreme conditions on biological nitrite oxidation was the aim of this study, using Nitrobacter agilis (ATCC 14123) as a model nitrifying bacterium. The initial O2 consumption rate of a concentrated cell suspension was used as a rapid assay to measure th...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1993-11, Vol.40 (2/3), p.442-448
Main Authors: Hunik, J.H, Meijer, H.J.G, Tramper, J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Mission oriented research
nitrification
Nitrobacter agilis
Nitrobacter winogradskyi
oxidation
Physiology and metabolism
salt
Sectie Proceskunde
Sub-department of Food and Bioprocess Engineering
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Summary:Measurement and description of the effects of extreme conditions on biological nitrite oxidation was the aim of this study, using Nitrobacter agilis (ATCC 14123) as a model nitrifying bacterium. The initial O2 consumption rate of a concentrated cell suspension was used as a rapid assay to measure the effects. Several relationships, based on Michaelis-Menten kinetics, were derived. These relationships describe the behaviour of N. agilis with respect to substrate inhibition, product inhibition and various salt concentrations up to 500 mmol.dm-3 with the pH ranging from 6.5 to 8.5. Substrate and product inhibition were pH-dependent and the substrate inhibition could be related to the undissociated nitrite. In contrast with previous reports on nitrite-oxidizing microorganisms, we did not observe severe inhibition by NH4+.
ISSN:0175-7598
1432-0614
DOI:10.1007/bf00170408