Simple determination of photosynthetic efficiency and photoinhibition of Dunaliella tertiolecta by saturating pulse fluorescence measurements

Saturating pulse fluorescence measurements, well known from studies of higher plants, were applied to cultures of the chlorophyte Dunaliella tertiolecta. Photochemical and non-photochemical quenching, and the photochemical yield and photochemical efficiency of photosystem II, were determined for cul...

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Bibliographic Details
Published in:Marine ecology. Progress series (Halstenbek) 1994-01, Vol.103 (1/2), p.187-196
Main Authors: Hofstraat, J. W., Peeters, J. C. H., Snel, J. F. H., Geel, C.
Format: Article
Language:English
Subjects:
Chlorophylls
Dunaliella
Dunaliella tertiolecta
Efficiency metrics
EPS
Fluorescence
Laboratorium voor Plantenfysiologie
Laboratory of Plant Physiology
Lighting
Luminous intensity
Marine
Photoinhibition
Photons
Phytoplankton
Plants
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Summary:Saturating pulse fluorescence measurements, well known from studies of higher plants, were applied to cultures of the chlorophyte Dunaliella tertiolecta. Photochemical and non-photochemical quenching, and the photochemical yield and photochemical efficiency of photosystem II, were determined for cultures of D. tertiolecta growing under varying light intensities. Photosynthetic efficiencies appeared to correlate well with growth rates determined for the D. tertiolecta cultures. The photochemical yield provides a measure of photoinhibition effects. The results indicate that the saturating pulse fluorescence method may be successfully used to determine photosynthetic characteristics of phytoplankton. The technique is simple to apply and can be adapted for photosynthetic measurements in situ, i.e. on phytoplankton in their natural habitat.
ISSN:0171-8630
1616-1599
DOI:10.3354/meps103187