Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: a resistance management strategy

Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua,...

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Bibliographic Details
Published in:Plant molecular biology 1994-10, Vol.26 (1), p.51-59
Main Authors: Salm, T. van der, Bosch, D, Honee, G, Feng, L, Munsterman, E, Bakker, P, Stiekema, W.J, Visser, B
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Bacillus thuringiensis
Bacillus thuringiensis - chemistry
Bacillus thuringiensis - genetics
Bacillus thuringiensis subsp. aizawai
Bacillus thuringiensis subsp. entomocidus
Bacillus thuringiensis Toxins
Bacterial Proteins - genetics
Bacterial Toxins
Base Sequence
cryia(b) gene
Endotoxins - genetics
Gene Expression Regulation, Plant
gene transfer
genetic resistance
Heliothis virescens
Hemolysin Proteins
insecticidal crystal proteins
Laboratorium voor Fytopathologie
Laboratorium voor Phytopathologie
Laboratory of Phytopathology
Lepidoptera
Manduca
Manduca sexta
Molecular Sequence Data
Nicotiana - genetics
Nicotiana tabacum
Pest Control, Biological
pest resistance
Plants, Genetically Modified
Plants, Toxic
recombinant DNA
Recombinant Fusion Proteins - biosynthesis
Solanum lycopersicum - genetics
Solanum lycopersicum var. lycopersicum
Solanum tuberosum
Spodoptera
Spodoptera exigua
structural genes
transgenic plants
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Summary:Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescens and Manduca sexta. The criteria used to modify both genes demonstrate that the removal of sequence motifs potentially resulting in premature polyadenylation and transcript instability causes increased insect resistance. The expression of a cryIC-cryIA(b) fusion resulting in protection against S. exigua, H. virescens and M. sexta demonstrates the potential of expressing translational fusions, not only to broaden the insect resistance of transgenic plants, but also to simultaneously employ different gene classes in resistance management strategies.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00039519