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Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: a resistance management strategy
Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua,...
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| Published in: | Plant molecular biology 1994-10, Vol.26 (1), p.51-59 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c387t-e79a5e3572ab477e1f0048d3c9477b5db860b14bebdb7a5ada53d24acbd8290c3 |
| container_end_page | 59 |
| container_issue | 1 |
| container_start_page | 51 |
| container_title | Plant molecular biology |
| container_volume | 26 |
| creator | Salm, T. van der Bosch, D Honee, G Feng, L Munsterman, E Bakker, P Stiekema, W.J Visser, B |
| description | Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescens and Manduca sexta. The criteria used to modify both genes demonstrate that the removal of sequence motifs potentially resulting in premature polyadenylation and transcript instability causes increased insect resistance. The expression of a cryIC-cryIA(b) fusion resulting in protection against S. exigua, H. virescens and M. sexta demonstrates the potential of expressing translational fusions, not only to broaden the insect resistance of transgenic plants, but also to simultaneously employ different gene classes in resistance management strategies. |
| doi_str_mv | 10.1007/BF00039519 |
| format | article |
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Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescens and Manduca sexta. The criteria used to modify both genes demonstrate that the removal of sequence motifs potentially resulting in premature polyadenylation and transcript instability causes increased insect resistance. The expression of a cryIC-cryIA(b) fusion resulting in protection against S. exigua, H. virescens and M. sexta demonstrates the potential of expressing translational fusions, not only to broaden the insect resistance of transgenic plants, but also to simultaneously employ different gene classes in resistance management strategies.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00039519</identifier><identifier>PMID: 7948900</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Amino Acid Sequence ; Animals ; Bacillus thuringiensis ; Bacillus thuringiensis - chemistry ; Bacillus thuringiensis - genetics ; Bacillus thuringiensis subsp. aizawai ; Bacillus thuringiensis subsp. entomocidus ; Bacillus thuringiensis Toxins ; Bacterial Proteins - genetics ; Bacterial Toxins ; Base Sequence ; cryia(b) gene ; Endotoxins - genetics ; Gene Expression Regulation, Plant ; gene transfer ; genetic resistance ; Heliothis virescens ; Hemolysin Proteins ; insecticidal crystal proteins ; Laboratorium voor Fytopathologie ; Laboratorium voor Phytopathologie ; Laboratory of Phytopathology ; Lepidoptera ; Manduca ; Manduca sexta ; Molecular Sequence Data ; Nicotiana - genetics ; Nicotiana tabacum ; Pest Control, Biological ; pest resistance ; Plants, Genetically Modified ; Plants, Toxic ; recombinant DNA ; Recombinant Fusion Proteins - biosynthesis ; Solanum lycopersicum - genetics ; Solanum lycopersicum var. lycopersicum ; Solanum tuberosum ; Spodoptera ; Spodoptera exigua ; structural genes ; transgenic plants</subject><ispartof>Plant molecular biology, 1994-10, Vol.26 (1), p.51-59</ispartof><rights>Wageningen University & Research</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-e79a5e3572ab477e1f0048d3c9477b5db860b14bebdb7a5ada53d24acbd8290c3</citedby><cites>FETCH-LOGICAL-c387t-e79a5e3572ab477e1f0048d3c9477b5db860b14bebdb7a5ada53d24acbd8290c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7948900$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salm, T. van der</creatorcontrib><creatorcontrib>Bosch, D</creatorcontrib><creatorcontrib>Honee, G</creatorcontrib><creatorcontrib>Feng, L</creatorcontrib><creatorcontrib>Munsterman, E</creatorcontrib><creatorcontrib>Bakker, P</creatorcontrib><creatorcontrib>Stiekema, W.J</creatorcontrib><creatorcontrib>Visser, B</creatorcontrib><title>Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: a resistance management strategy</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescens and Manduca sexta. The criteria used to modify both genes demonstrate that the removal of sequence motifs potentially resulting in premature polyadenylation and transcript instability causes increased insect resistance. The expression of a cryIC-cryIA(b) fusion resulting in protection against S. exigua, H. virescens and M. sexta demonstrates the potential of expressing translational fusions, not only to broaden the insect resistance of transgenic plants, but also to simultaneously employ different gene classes in resistance management strategies.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bacillus thuringiensis</subject><subject>Bacillus thuringiensis - chemistry</subject><subject>Bacillus thuringiensis - genetics</subject><subject>Bacillus thuringiensis subsp. aizawai</subject><subject>Bacillus thuringiensis subsp. entomocidus</subject><subject>Bacillus thuringiensis Toxins</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Toxins</subject><subject>Base Sequence</subject><subject>cryia(b) gene</subject><subject>Endotoxins - genetics</subject><subject>Gene Expression Regulation, Plant</subject><subject>gene transfer</subject><subject>genetic resistance</subject><subject>Heliothis virescens</subject><subject>Hemolysin Proteins</subject><subject>insecticidal crystal proteins</subject><subject>Laboratorium voor Fytopathologie</subject><subject>Laboratorium voor Phytopathologie</subject><subject>Laboratory of Phytopathology</subject><subject>Lepidoptera</subject><subject>Manduca</subject><subject>Manduca sexta</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana tabacum</subject><subject>Pest Control, Biological</subject><subject>pest resistance</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Toxic</subject><subject>recombinant DNA</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Solanum lycopersicum - genetics</subject><subject>Solanum lycopersicum var. lycopersicum</subject><subject>Solanum tuberosum</subject><subject>Spodoptera</subject><subject>Spodoptera exigua</subject><subject>structural genes</subject><subject>transgenic plants</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNpNkUFv1DAQhS0EKtvChTvCJwRIATtO4qS3dkVhpUocoGdrbE-CUeIstqOyf4LfXIddARePRu-bN7YfIS84e88Zkx-ubxhjoqt594hseC1FUbOyfUw2jDeyqCpePiXnMf5gLOOiOSNnsqvajrEN-b3zEU2iAaOLCbxBOvc0BfBxQO8M3Y_gU6TpOySKv_aZi3SaresdWnoNxo3jsspLcH5w6LMNNeGwu3qj31Lw9k-zpdkM4yWF_xdN4GHACX2iMW9MOByekSc9jBGfn-oFubv5-G37ubj98mm3vbotjGhlKlB2UKOoZQm6khJ5z1jVWmG63Ona6rZhmlcatdUSarBQC1tWYLRty44ZcUG6o-89rM_0-VAegnFRzeDU6HSAcFD3S1B-XMt-0VGVsmnaPPv6OLsP888FY1KTiwbH_FE4L1HxpilbyUQG3x1BE-YYA_ZqH9y0-nKm1uDUv-Ay_PLkuugJ7V_0lFTWXx31HmYFQ8g3vftaMi4YrzpZcy4eAJWLoLU</recordid><startdate>19941001</startdate><enddate>19941001</enddate><creator>Salm, T. van der</creator><creator>Bosch, D</creator><creator>Honee, G</creator><creator>Feng, L</creator><creator>Munsterman, E</creator><creator>Bakker, P</creator><creator>Stiekema, W.J</creator><creator>Visser, B</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>QVL</scope></search><sort><creationdate>19941001</creationdate><title>Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: a resistance management strategy</title><author>Salm, T. van der ; Bosch, D ; Honee, G ; Feng, L ; Munsterman, E ; Bakker, P ; Stiekema, W.J ; Visser, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-e79a5e3572ab477e1f0048d3c9477b5db860b14bebdb7a5ada53d24acbd8290c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bacillus thuringiensis</topic><topic>Bacillus thuringiensis - chemistry</topic><topic>Bacillus thuringiensis - genetics</topic><topic>Bacillus thuringiensis subsp. aizawai</topic><topic>Bacillus thuringiensis subsp. entomocidus</topic><topic>Bacillus thuringiensis Toxins</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Toxins</topic><topic>Base Sequence</topic><topic>cryia(b) gene</topic><topic>Endotoxins - genetics</topic><topic>Gene Expression Regulation, Plant</topic><topic>gene transfer</topic><topic>genetic resistance</topic><topic>Heliothis virescens</topic><topic>Hemolysin Proteins</topic><topic>insecticidal crystal proteins</topic><topic>Laboratorium voor Fytopathologie</topic><topic>Laboratorium voor Phytopathologie</topic><topic>Laboratory of Phytopathology</topic><topic>Lepidoptera</topic><topic>Manduca</topic><topic>Manduca sexta</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana tabacum</topic><topic>Pest Control, Biological</topic><topic>pest resistance</topic><topic>Plants, Genetically Modified</topic><topic>Plants, Toxic</topic><topic>recombinant DNA</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Solanum lycopersicum - genetics</topic><topic>Solanum lycopersicum var. lycopersicum</topic><topic>Solanum tuberosum</topic><topic>Spodoptera</topic><topic>Spodoptera exigua</topic><topic>structural genes</topic><topic>transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salm, T. van der</creatorcontrib><creatorcontrib>Bosch, D</creatorcontrib><creatorcontrib>Honee, G</creatorcontrib><creatorcontrib>Feng, L</creatorcontrib><creatorcontrib>Munsterman, E</creatorcontrib><creatorcontrib>Bakker, P</creatorcontrib><creatorcontrib>Stiekema, W.J</creatorcontrib><creatorcontrib>Visser, B</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>NARCIS:Publications</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salm, T. van der</au><au>Bosch, D</au><au>Honee, G</au><au>Feng, L</au><au>Munsterman, E</au><au>Bakker, P</au><au>Stiekema, W.J</au><au>Visser, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: a resistance management strategy</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1994-10-01</date><risdate>1994</risdate><volume>26</volume><issue>1</issue><spage>51</spage><epage>59</epage><pages>51-59</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescens and Manduca sexta. The criteria used to modify both genes demonstrate that the removal of sequence motifs potentially resulting in premature polyadenylation and transcript instability causes increased insect resistance. The expression of a cryIC-cryIA(b) fusion resulting in protection against S. exigua, H. virescens and M. sexta demonstrates the potential of expressing translational fusions, not only to broaden the insect resistance of transgenic plants, but also to simultaneously employ different gene classes in resistance management strategies.</abstract><cop>Netherlands</cop><pmid>7948900</pmid><doi>10.1007/BF00039519</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1994-10, Vol.26 (1), p.51-59 |
| issn | 0167-4412 1573-5028 |
| language | eng |
| recordid | cdi_wageningen_narcis_oai_library_wur_nl_wurpubs_27668 |
| source | Springer LINK Archives |
| subjects | Amino Acid Sequence Animals Bacillus thuringiensis Bacillus thuringiensis - chemistry Bacillus thuringiensis - genetics Bacillus thuringiensis subsp. aizawai Bacillus thuringiensis subsp. entomocidus Bacillus thuringiensis Toxins Bacterial Proteins - genetics Bacterial Toxins Base Sequence cryia(b) gene Endotoxins - genetics Gene Expression Regulation, Plant gene transfer genetic resistance Heliothis virescens Hemolysin Proteins insecticidal crystal proteins Laboratorium voor Fytopathologie Laboratorium voor Phytopathologie Laboratory of Phytopathology Lepidoptera Manduca Manduca sexta Molecular Sequence Data Nicotiana - genetics Nicotiana tabacum Pest Control, Biological pest resistance Plants, Genetically Modified Plants, Toxic recombinant DNA Recombinant Fusion Proteins - biosynthesis Solanum lycopersicum - genetics Solanum lycopersicum var. lycopersicum Solanum tuberosum Spodoptera Spodoptera exigua structural genes transgenic plants |
| title | Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: a resistance management strategy |
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