Development of a bioassay to test phloem sap samples from lettuce for resistance to Nasonovia ribisnigri (Homoptera, Aphididae)

The Nr-gene-based resistance of lettuce to the aphid Nasonovia ribisnigri (Mosley) has previously been shown to be located in the phloem. Since chemical analyses of the phloem sap had shown no differences between resistant and susceptible lines, a bioassay was developed in order to test samples from...

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Bibliographic Details
Published in:Journal of chemical ecology 1995-06, Vol.21 (6), p.761-774
Main Authors: Helden, M. van (Wageningen Agricultural University, Wageningen, The Netherlands.), Heest, H.P.N.F. van, Beek, T.A. van, Tjallingii, W.F
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Aphididae
Biological and medical sciences
BRASSICA NAPUS
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DOSAGE BIOLOGIQUE
ENSAYO BIOLOGICO
EPS
FLOEMA
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
Homoptera
Laboratorium voor Entomologie
Laboratory of Entomology
LACTUCA SATIVA
MACROSIPHUM EUPHORBIAE
MIELADA
MIELLAT
NASONOVIA RIBISNIGRI
Pest animals
Pest resistance
PHLOEME
RESISTANCE AUX ORGANISMES NUISIBLES
RESISTENCIA A LAS PLAGAS
SAVIA
SEVE
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Varietal selection. Specialized plant breeding, plant breeding aims
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Summary:The Nr-gene-based resistance of lettuce to the aphid Nasonovia ribisnigri (Mosley) has previously been shown to be located in the phloem. Since chemical analyses of the phloem sap had shown no differences between resistant and susceptible lines, a bioassay was developed in order to test samples from resistant and susceptible plants on aphid feeding. For this, whole-plant extracts, honeydew, and EDTA-collected phloem extracts were obtained, and a sensitive bioassay was developed using EDTA samples. The EDTA was removed, and samples were added to a simple sucrose solution or to a complex artificial diet and presented in a choice situation comparing extracts from resistant and susceptible plants. EDTA-collected phloem sap samples from susceptible plants were preferred to those from resistant plants. The resistance is probably based on a feeding deterrent activity of the phloem sap in the resistant plant
ISSN:0098-0331
1573-1561
DOI:10.1007/BF02033459