PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures

The size of the internal transcribed spacer (ITS) region as measured by gel electrophoresis of PCR products, amplified by primers ITS1 and ITS4, was over 800 bp for all Saccharomyces sensu stricto species, but yeasts belonging to other Saccharomyces species had a shorter ITS region, making this char...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1996-04, Vol.137 (2/3), p.253-256
Main Authors: Valente, P, Gouveia, F.C, Lemos, G.A. de, Pimentel, D, Elsas, J.D. van, Mendonca-Hagler, L.C, Hagler, A.N
Format: Article
Language:English
Subjects:
Amplification
Base Sequence
Biological and medical sciences
chemotaxonomy
differentiation
DNA Primers - genetics
DNA, Fungal - genetics
DNA, Ribosomal - genetics
Electrophoresis
Fundamental and applied biological sciences. Psychology
Gel electrophoresis
Genes. Genome
Instituut voor Plantenziektenkundig Onderzoek
Internal transcribed spacer
Microbiology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mycology
PCR
Polymerase Chain Reaction
Research Institute for Plant Protection
ribosomal DNA
Saccharomyces
Saccharomyces - classification
Saccharomyces - genetics
Saccharomyces cerevisiae - classification
Saccharomyces cerevisiae - genetics
Spacer region
Species
Species Specificity
Systematics
Yeast taxonomy
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The size of the internal transcribed spacer (ITS) region as measured by gel electrophoresis of PCR products, amplified by primers ITS1 and ITS4, was over 800 bp for all Saccharomyces sensu stricto species, but yeasts belonging to other Saccharomyces species had a shorter ITS region, making this characteristic potentially useful in the identification of Saccharomyces isolates. The ITS product length was homogeneous within the species Saccharomyces cerevisiae.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1996.tb08114.x