Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures

• Published in: The Plant cell 1997-12, Vol.9 (12), p.2119-2134
• Main Authors: Barthels, N, Lee, F.M. van der, Klap, J, Goddijn, O.J.M, Karimi, M, Puzio, P, Grundler, F.M.W, Ohl, S.A, Lindsey, K, Robertson, L
• Format: Article
• Language: English
• Subjects: ADN RECOMBINADO; ADN RECOMBINE; AGALLAS; Agrotechnological Research Institute; Animals; Arabidopsis - genetics; Arabidopsis - parasitology; ARABIDOPSIS THALIANA; Base Sequence; BETA-GLUCURONIDASE; BIOCHEMISTRY; BIOCHIMIE; BIOQUIMICA; CELLS; CELLULE; CELULAS; DNA Primers - genetics; DNA, Bacterial - genetics; EXPRESION GENICA; EXPRESSION DES GENES; GALLE; GENE; GENE EXPRESSION; Gene Expression Regulation, Plant; GENES; Genes, Plant; Genes, Regulator; Genes, Reporter; GENETIC TRANSFORMATION; Genetic Vectors; Giant cells; GLICOSIDASAS; Glucuronidase - genetics; GLYCOSIDASE; GLYCOSIDASES; HETERODERA SCHACHTII; HISTOENZYMOLOGY; INFECTIONS; Inoculation; Instituut voor Agrotechnologisch Onderzoek; MELOIDOGYNE INCOGNITA; METHODE; METHODS; METODOS; Nematoda - pathogenicity; Nematodes; PATHOGENESE; PATHOGENESIS; PATOGENESIS; Plant cells; Plant Diseases - genetics; Plant Diseases - parasitology; PLANT GALLS; Plant parasitic nematodes; Plant roots; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants; Plants, Genetically Modified; PLASMID VECTORS; Polymerase Chain Reaction; Promoter Regions, Genetic; PROMOTER TAGGING; PROMOTERS; RACINE; RAICES; RECOMBINANT DNA; Reporter genes; ROOTS; SYNCYTIA; TEMPORAL VARIATION; Transcriptional Activation; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; TRANSGENIC PLANTS; XIPHINEMA DIVERSICAUDATUM
• ISSN: 1040-4651; 1532-298X
• DOI: 10.1105/tpc.9.12.2119

In the quest for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures, we show that promoter tagging is a valuable tool. A large collection of transgenic Arabidopsis plants was generated. They were transformed with a beta-glucuronidase gene functioning as a promoter tag. Three T-DNA constructs, pGV1047, pdeltagusBin19, and pMOG553, were used. Early responses to nematode invasion were of primary interest. Six lines exhibiting beta-glucuronidase activity in syncytia induced by the beet cyst nematode were studied. Reporter gene activation was also identified in galls induced by root knot and ectoparasitic nematodes. Time-course studies revealed that all six tags were differentially activated during the development of the feeding structure. T-DNA-flanking regions responsible for the observed responses after nematode infection were isolated and characterized for promoter activity