Somatic embryogenesis and plant regeneration in Lilium longiflorum Thunb

Friable callus was obtained from styles and flower pedicels of Lilium longiflorum Snow Queen and the Oriental lily hybrid Star Gazer on Murashige and Skoog (MS) media containing either 2 micromolar dicamba or 2 micromolar picloram. Cell suspension cultures were established by suspending the callus o...

Full description

Saved in:
Bibliographic Details
Published in:Plant cell reports 1997-12, Vol.17 (2), p.113-118
Main Authors: Tribulato, A, Remotti, P.C, Loffler, H.J.M, Tuyl, J.M. van
Format: Article
Language:English
Subjects:
2,4-D
Bacteria
benzyladenine
Biological and medical sciences
Biotechnology
bulbs
callus
cell differentiation
cell suspension culture
Centrum voor Plantenveredelings- en Reproduktieonderzoek
culture media
developmental stages
dicamba
dose response
embryo (plant)
Embryonic growth stage
Embryos
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
histology
In vitro propagation: entire plant regeneration from tissues and cell cultures
Lilium longiflorum
methodology
Methods. Procedures. Technologies
murashige and skoog medium
naphthaleneacetic acid
Picloram
plant anatomy
Plant cells and fungal cells
Plant physiology and development
regenerative ability
somatic embryogenesis
Tissue cultures, protoplasts
ultrastructure
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Friable callus was obtained from styles and flower pedicels of Lilium longiflorum Snow Queen and the Oriental lily hybrid Star Gazer on Murashige and Skoog (MS) media containing either 2 micromolar dicamba or 2 micromolar picloram. Cell suspension cultures were established by suspending the callus of L. longiflorum Snow Queen in liquid medium containing 2 micromolar dicamba. Through a purification process, a fine fast-growing cell suspension was obtained. This suspension was composed of a homogenous population of small dense cells, which tended to organise into embryo like structures (ELS). In liquid culture with the auxin dicamba, the ELS underwent continuous callus formation. When transferred to solidified hormone-free MS medium, the ELS germinated, forming complete plantlets. Histological investigation showed that in the ELS both shoot and root meristems were distinctly evident. It was concluded that the ELS obtained were in fact somatic embryos.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050362