Microsatellites of the parasitic nematode Haemonchus contortus: polymorphism and linkage with a direct repeat

To develop tools to analyse parasitic nematode population structures and the effects of selection pressure on the nematode population, we isolated and characterised 13 microsatellite markers of the nematode Haemonchus contortus. The density of CA/GT microsatellites, once in 575 kb, is lower than in...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 1997-10, Vol.89 (1), p.97-107
Main Authors: Hoekstra, Ruurdtje, Criado-Fornelio, Angel, Fakkeldij, Jeroen, Bergman, Janske, Roos, Marleen H
Format: Article
Language:English
Subjects:
animal parasitic nematodes
Animals
Base Sequence
Dinucleotide Repeats
DNA, Helminth - isolation & purification
Drug resistance
genbank/u84474
genbank/u84475
genbank/u84476
genbank/u84477
genbank/u84478
genbank/u84479
genbank/u84480
genbank/u84481
genbank/u84482
genbank/u84483
genbank/u84484
genbank/u84485
genbank/u84486
genbank/u86701
gene frequency
Genetic Linkage
genetic markers
genetic polymorphism
genetic variation
Haemonchus - genetics
Haemonchus contortus
ID Lelystad, Institute for Animal Science and Health
ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid
linkage (genetics)
Microsatellite
Microsatellite Repeats
Molecular Sequence Data
Nematode
nucleotide sequences
Polymorphism, Genetic
repetitive sequences
Sequence Alignment
Sequence Homology, Nucleic Acid
Tricostrongylus colubriformis
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Description
Summary:To develop tools to analyse parasitic nematode population structures and the effects of selection pressure on the nematode population, we isolated and characterised 13 microsatellite markers of the nematode Haemonchus contortus. The density of CA/GT microsatellites, once in 575 kb, is lower than in mammals. Although the isolated CA/GT repeats were imperfect, the majority of the microsatellites were polymorphic in one or more populations. An extensive genetic diversity both within and between populations could be established. Two-thirds of the CA/GT microsatellites were followed by a variable number of 128 bp direct repeat elements, HcREP1. HcREP1 is a repetitive element in the H. contortus genome, which is homologous to the repetitive TcREP element in the nematode Trichostrongylus colubriformis.
ISSN:0166-6851
1872-9428
DOI:10.1016/S0166-6851(97)00108-4