Porcine myelomonocytic markers: summary of the second international swine CD workshop

Forty five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. As a result of these analyses, a new swine workshop cluster, SWC9, compos...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 1998-01, Vol.60 (3), p.329-341
Main Authors: Domı́nguez, J, Ezquerra, A, Alonso, F, McCullough, K, Summerfield, A, Bianchi, A, Zwart, R.J, Kim, Y.B, Blecha, F, Eicher, S, Murtaugh, M, Pampusch, M, Burger, K
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - metabolism
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
ANTIGEN ANTIBODY REACTIONS
ANTIGENE
ANTIGENOS
ANTIGENS
Antigens, Differentiation, Myelomonocytic - immunology
Biomarkers
CD14 mAbs
CERDO
Flow Cytometry
Granulocytes - immunology
Humans
ID Lelystad, Institute for Animal Science and Health
ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid
Immunohistochemistry
MACROFAGOS
MACROPHAGE
MACROPHAGES
Macrophages - immunology
MONOCLONAL ANTIBODIES
Monocytes - immunology
PORCIN
Porcine myelomonocytic markers
REACCION ANTIGENO ANTICUERPO
REACTION ANTIGENE ANTICORPS
SWINE
Swine - immunology
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Summary:Forty five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. As a result of these analyses, a new swine workshop cluster, SWC9, composed of two mAbs that recognize an antigen selectively expressed on mature macrophages, was defined. In addition, several mAbs were identified, allowing the differentiation of granulocytes from monocytes/macrophages, or monocytes from macrophages. Further work is required to identify the antigen recognized by these mAbs. Nevertheless, they should already prove useful for the identification of different stages in the macrophage maturation/differentiation, and will certainly aid analyses on the complexity of the mononuclear phagocyte system in the pig. Finally, the cross-reactivity of three anti-human CD14 mAbs with porcine myelomonocytic cells was established in this workshop.
ISSN:0165-2427
1873-2534
DOI:10.1016/S0165-2427(97)00109-8