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Porcine myelomonocytic markers: summary of the second international swine CD workshop
Forty five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. As a result of these analyses, a new swine workshop cluster, SWC9, compos...
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| Published in: | Veterinary immunology and immunopathology 1998-01, Vol.60 (3), p.329-341 |
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| Main Authors: | , , , , , , , , , , , , |
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| container_end_page | 341 |
| container_issue | 3 |
| container_start_page | 329 |
| container_title | Veterinary immunology and immunopathology |
| container_volume | 60 |
| creator | Domı́nguez, J Ezquerra, A Alonso, F McCullough, K Summerfield, A Bianchi, A Zwart, R.J Kim, Y.B Blecha, F Eicher, S Murtaugh, M Pampusch, M Burger, K |
| description | Forty five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. As a result of these analyses, a new swine workshop cluster, SWC9, composed of two mAbs that recognize an antigen selectively expressed on mature macrophages, was defined. In addition, several mAbs were identified, allowing the differentiation of granulocytes from monocytes/macrophages, or monocytes from macrophages. Further work is required to identify the antigen recognized by these mAbs. Nevertheless, they should already prove useful for the identification of different stages in the macrophage maturation/differentiation, and will certainly aid analyses on the complexity of the mononuclear phagocyte system in the pig. Finally, the cross-reactivity of three anti-human CD14 mAbs with porcine myelomonocytic cells was established in this workshop. |
| doi_str_mv | 10.1016/S0165-2427(97)00109-8 |
| format | article |
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As a result of these analyses, a new swine workshop cluster, SWC9, composed of two mAbs that recognize an antigen selectively expressed on mature macrophages, was defined. In addition, several mAbs were identified, allowing the differentiation of granulocytes from monocytes/macrophages, or monocytes from macrophages. Further work is required to identify the antigen recognized by these mAbs. Nevertheless, they should already prove useful for the identification of different stages in the macrophage maturation/differentiation, and will certainly aid analyses on the complexity of the mononuclear phagocyte system in the pig. Finally, the cross-reactivity of three anti-human CD14 mAbs with porcine myelomonocytic cells was established in this workshop.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/S0165-2427(97)00109-8</identifier><identifier>PMID: 9589571</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies, Monoclonal - metabolism ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; ANTIGEN ANTIBODY REACTIONS ; ANTIGENE ; ANTIGENOS ; ANTIGENS ; Antigens, Differentiation, Myelomonocytic - immunology ; Biomarkers ; CD14 mAbs ; CERDO ; Flow Cytometry ; Granulocytes - immunology ; Humans ; ID Lelystad, Institute for Animal Science and Health ; ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid ; Immunohistochemistry ; MACROFAGOS ; MACROPHAGE ; MACROPHAGES ; Macrophages - immunology ; MONOCLONAL ANTIBODIES ; Monocytes - immunology ; PORCIN ; Porcine myelomonocytic markers ; REACCION ANTIGENO ANTICUERPO ; REACTION ANTIGENE ANTICORPS ; SWINE ; Swine - immunology</subject><ispartof>Veterinary immunology and immunopathology, 1998-01, Vol.60 (3), p.329-341</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>Wageningen University & Research</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-fdd3bbe91061cec53bb29dbc952214d406b2444023aff481fd9a48b12f588e23</citedby><cites>FETCH-LOGICAL-c435t-fdd3bbe91061cec53bb29dbc952214d406b2444023aff481fd9a48b12f588e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,309,310,314,780,784,789,790,885,23928,23929,25138,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9589571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Domı́nguez, J</creatorcontrib><creatorcontrib>Ezquerra, A</creatorcontrib><creatorcontrib>Alonso, F</creatorcontrib><creatorcontrib>McCullough, K</creatorcontrib><creatorcontrib>Summerfield, A</creatorcontrib><creatorcontrib>Bianchi, A</creatorcontrib><creatorcontrib>Zwart, R.J</creatorcontrib><creatorcontrib>Kim, Y.B</creatorcontrib><creatorcontrib>Blecha, F</creatorcontrib><creatorcontrib>Eicher, S</creatorcontrib><creatorcontrib>Murtaugh, M</creatorcontrib><creatorcontrib>Pampusch, M</creatorcontrib><creatorcontrib>Burger, K</creatorcontrib><title>Porcine myelomonocytic markers: summary of the second international swine CD workshop</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>Forty five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. As a result of these analyses, a new swine workshop cluster, SWC9, composed of two mAbs that recognize an antigen selectively expressed on mature macrophages, was defined. In addition, several mAbs were identified, allowing the differentiation of granulocytes from monocytes/macrophages, or monocytes from macrophages. Further work is required to identify the antigen recognized by these mAbs. Nevertheless, they should already prove useful for the identification of different stages in the macrophage maturation/differentiation, and will certainly aid analyses on the complexity of the mononuclear phagocyte system in the pig. Finally, the cross-reactivity of three anti-human CD14 mAbs with porcine myelomonocytic cells was established in this workshop.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - metabolism</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>ANTIGEN ANTIBODY REACTIONS</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>ANTIGENS</subject><subject>Antigens, Differentiation, Myelomonocytic - immunology</subject><subject>Biomarkers</subject><subject>CD14 mAbs</subject><subject>CERDO</subject><subject>Flow Cytometry</subject><subject>Granulocytes - immunology</subject><subject>Humans</subject><subject>ID Lelystad, Institute for Animal Science and Health</subject><subject>ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid</subject><subject>Immunohistochemistry</subject><subject>MACROFAGOS</subject><subject>MACROPHAGE</subject><subject>MACROPHAGES</subject><subject>Macrophages - immunology</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>Monocytes - immunology</subject><subject>PORCIN</subject><subject>Porcine myelomonocytic markers</subject><subject>REACCION ANTIGENO ANTICUERPO</subject><subject>REACTION ANTIGENE ANTICORPS</subject><subject>SWINE</subject><subject>Swine - immunology</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFUU1v1DAQtRCoLIWfUMknBIeA7dgbuxeElk9pBUiU88hxJq1pYm_thNX-e5zuqhy5zNh6743H7xFywdkbzvj67c9SVCWkaF6Z5jVjnJlKPyIrrpu6EqqWj8nqgfKUPMv5N2NMGa3PyJlR2qiGr8ivHzE5H5COBxziGEN0h8k7Otp0iylf0jyP5XygsafTDdKMLoaO-jBhCnbyMdiB5v0yYfOB7mO6zTdx95w86e2Q8cWpn5OrTx-vNl-q7ffPXzfvt5WTtZqqvuvqtkXD2Zo7dKpchOlaZ5QQXHaSrVshpWSitn0vNe87Y6VuueiV1ijqc3J5HLu31xh8KAWCLf_JEK2HwbeprA77OUEYlrab2ww1a0xtivjlUbxL8W7GPMHos8NhsAHjnKEpTvFG8UJUR6JLMeeEPeySXzwBzmCJAu6jgMVnMA3cRwG66C5OD8ztiN2D6uT9P7y3Eex1Klt_23JjNGPC8AV_d8SxOPjHY4LsPAaHnU_oJuii_88GfwGC9aRH</recordid><startdate>19980130</startdate><enddate>19980130</enddate><creator>Domı́nguez, J</creator><creator>Ezquerra, A</creator><creator>Alonso, F</creator><creator>McCullough, K</creator><creator>Summerfield, A</creator><creator>Bianchi, A</creator><creator>Zwart, R.J</creator><creator>Kim, Y.B</creator><creator>Blecha, F</creator><creator>Eicher, S</creator><creator>Murtaugh, M</creator><creator>Pampusch, M</creator><creator>Burger, K</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>QVL</scope></search><sort><creationdate>19980130</creationdate><title>Porcine myelomonocytic markers: summary of the second international swine CD workshop</title><author>Domı́nguez, J ; Ezquerra, A ; Alonso, F ; McCullough, K ; Summerfield, A ; Bianchi, A ; Zwart, R.J ; Kim, Y.B ; Blecha, F ; Eicher, S ; Murtaugh, M ; Pampusch, M ; Burger, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-fdd3bbe91061cec53bb29dbc952214d406b2444023aff481fd9a48b12f588e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - metabolism</topic><topic>ANTICORPS MONOCLONAL</topic><topic>ANTICUERPOS MONOCLONALES</topic><topic>ANTIGEN ANTIBODY REACTIONS</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>ANTIGENS</topic><topic>Antigens, Differentiation, Myelomonocytic - immunology</topic><topic>Biomarkers</topic><topic>CD14 mAbs</topic><topic>CERDO</topic><topic>Flow Cytometry</topic><topic>Granulocytes - immunology</topic><topic>Humans</topic><topic>ID Lelystad, Institute for Animal Science and Health</topic><topic>ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid</topic><topic>Immunohistochemistry</topic><topic>MACROFAGOS</topic><topic>MACROPHAGE</topic><topic>MACROPHAGES</topic><topic>Macrophages - immunology</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>Monocytes - immunology</topic><topic>PORCIN</topic><topic>Porcine myelomonocytic markers</topic><topic>REACCION ANTIGENO ANTICUERPO</topic><topic>REACTION ANTIGENE ANTICORPS</topic><topic>SWINE</topic><topic>Swine - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Domı́nguez, J</creatorcontrib><creatorcontrib>Ezquerra, A</creatorcontrib><creatorcontrib>Alonso, F</creatorcontrib><creatorcontrib>McCullough, K</creatorcontrib><creatorcontrib>Summerfield, A</creatorcontrib><creatorcontrib>Bianchi, A</creatorcontrib><creatorcontrib>Zwart, R.J</creatorcontrib><creatorcontrib>Kim, Y.B</creatorcontrib><creatorcontrib>Blecha, F</creatorcontrib><creatorcontrib>Eicher, S</creatorcontrib><creatorcontrib>Murtaugh, M</creatorcontrib><creatorcontrib>Pampusch, M</creatorcontrib><creatorcontrib>Burger, K</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>NARCIS:Publications</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Domı́nguez, J</au><au>Ezquerra, A</au><au>Alonso, F</au><au>McCullough, K</au><au>Summerfield, A</au><au>Bianchi, A</au><au>Zwart, R.J</au><au>Kim, Y.B</au><au>Blecha, F</au><au>Eicher, S</au><au>Murtaugh, M</au><au>Pampusch, M</au><au>Burger, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porcine myelomonocytic markers: summary of the second international swine CD workshop</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>1998-01-30</date><risdate>1998</risdate><volume>60</volume><issue>3</issue><spage>329</spage><epage>341</epage><pages>329-341</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>Forty five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. 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| ispartof | Veterinary immunology and immunopathology, 1998-01, Vol.60 (3), p.329-341 |
| issn | 0165-2427 1873-2534 |
| language | eng |
| recordid | cdi_wageningen_narcis_oai_library_wur_nl_wurpubs_307939 |
| source | ScienceDirect Journals |
| subjects | Animals Antibodies, Monoclonal - metabolism ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES ANTIGEN ANTIBODY REACTIONS ANTIGENE ANTIGENOS ANTIGENS Antigens, Differentiation, Myelomonocytic - immunology Biomarkers CD14 mAbs CERDO Flow Cytometry Granulocytes - immunology Humans ID Lelystad, Institute for Animal Science and Health ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid Immunohistochemistry MACROFAGOS MACROPHAGE MACROPHAGES Macrophages - immunology MONOCLONAL ANTIBODIES Monocytes - immunology PORCIN Porcine myelomonocytic markers REACCION ANTIGENO ANTICUERPO REACTION ANTIGENE ANTICORPS SWINE Swine - immunology |
| title | Porcine myelomonocytic markers: summary of the second international swine CD workshop |
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