Identification of metabolites of the anabolic steroid methandienone formed by bovine hepatocytes in vitro

Monolayer cultures of bovine hepatocytes were used to investigate the biotransformation of methandienone in vitro. After incubation of bovine hepatocytes with methandienone, samples were taken at different times. The samples were treated with deconjugation enzymes and extracted with diethyl ether. T...

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Bibliographic Details
Published in:Analyst (London) 1998, Vol.123 (12), p.2637-2641
Main Authors: HOOIJERINK, D, SCHILT, R, HOOGENBOOM, R, HUVENEERS-OORSPRONG, M
Format: Article
Language:English
Subjects:
Anabolic Agents - analysis
Anabolic Agents - chemistry
Anabolic Agents - metabolism
Animal productions
Animals
Biological and medical sciences
Biotransformation
Cattle - metabolism
Cells, Cultured
Chromatography, High Pressure Liquid
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
Liver - metabolism
Male
Methandrostenolone - analogs & derivatives
Methandrostenolone - analysis
Methandrostenolone - chemistry
Methandrostenolone - metabolism
RIKILT
Terrestrial animal productions
Vertebrates
Wageningen Food Safety Research
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Summary:Monolayer cultures of bovine hepatocytes were used to investigate the biotransformation of methandienone in vitro. After incubation of bovine hepatocytes with methandienone, samples were taken at different times. The samples were treated with deconjugation enzymes and extracted with diethyl ether. The metabolites formed were converted to their trimethylsilylether derivatives. By using gas chromatography-mass spectrometry with electron impact and chemical ionisation, several metabolites were identified. After 24 h of incubation with bovine hepatocytes, 83% of the parent compound was converted to its metabolites. The major metabolite found was 6-beta-hydroxymethandienone with a yield of 24%. This compound was identified after comparison with an authentic sample of 6 beta-hydroxymethandienone, which was synthesized chemically.
ISSN:0003-2654
1364-5528
DOI:10.1039/a805132a