Testing of Raw Milk for Tetracycline Residues

A newly improved Bacillus calidolactis tube diffusion test and two postscreening test systems—a receptor assay (Charm HVS; Charm Sciences, Inc., Malden, MA) and a newly developed Bacillus cereus ATCC 11778 mycoides test system—were evaluated for the detection and identification of tetracycline resid...

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Bibliographic Details
Published in:Journal of dairy science 1998-09, Vol.81 (9), p.2341-2345
Main Authors: Nouws, Jacques F.M., Loeffen, Gerard, Schouten, Jan, Van Egmond, Harry, Keukens, Henk, Stegeman, Henk
Format: Article
Language:English
Subjects:
Animals
ANTIBIOTICOS
ANTIBIOTICS
ANTIBIOTIQUE
ANTIPROTOZOAIRE
ANTIPROTOZOAL AGENTS
Bacillus
BACILLUS CEREUS
BACILLUS STEAROTHERMOPHILUS
BACILLUS STEAROTHERMOPHILUS VAR. CALIDOL
bioassay
BIOASSAYS
Biological and medical sciences
Charm HVS test
CHLORTETRACYCLINE
Chlortetracycline - analysis
Chromatography, High Pressure Liquid
DETECTION
DIFFUSION
DIFUSION
DOXYCYCLINE
Doxycycline - analysis
Drug Residues - analysis
ENSAYO
ENSAYO BIOLOGICO
Food industries
Fundamental and applied biological sciences. Psychology
INHIBICION
INHIBITION
LAIT
LECHE
MEDICAMENTOS CONTRA PROTOZOARIOS
MILK
Milk - chemistry
Milk and cheese industries. Ice creams
NETHERLANDS
OXITETRACICLINA
OXYTETRACYCLINE
Oxytetracycline - analysis
PAISES BAJOS
PAYS BAS
RESIDU
RESIDUES
residues in milk
RESIDUOS
RIKILT
TEST BIOLOGIQUE
TESTAGE
TESTING
TETRACICLINA
TETRACYCLINE
Tetracycline - analysis
TETRACYCLINES
Wageningen Food Safety Research
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Summary:A newly improved Bacillus calidolactis tube diffusion test and two postscreening test systems—a receptor assay (Charm HVS; Charm Sciences, Inc., Malden, MA) and a newly developed Bacillus cereus ATCC 11778 mycoides test system—were evaluated for the detection and identification of tetracycline residues using 973 samples of bulk milk taken at random in The Netherlands. All milk samples were assayed with the B. calidolactis tube and the receptor test. The milk samples testing as suspect or positive with one or both of the test systems were analyzed with HPLC (limit of detection, 10ng/ml) and the recently developed B. cereus test system. The B. calidolactis tube diffusion test detected tetracycline residues >45ng/ml in milk. With the B. cereus test plate, residues of oxytetracycline and tetracycline of >30ng/ml milk were detected; for chlortetracycline and doxycycline, the detection limit was 10ng/ml. Raw milk exhibiting inhibition diameters of
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(98)70124-9