Characterisation of a polymorphic Tc1-like transposable element of the parasitic nematode Haemonchus contortus

Hctc1, a member of the Tc1-family of transposable elements was isolated from the parasitic nematode Haemonchus contortus. Hctc1 is 1590 bp long, is flanked by 55 bp inverted repeats and carries a single open reading frame of a 340 amino acid transposase-like protein. Hctc1 is similar to Tc1 of Caeno...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 1999-07, Vol.102 (1), p.157-166
Main Authors: Hoekstra, R., Otsen, M., Lenstra, J.A., Roos, M.H.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
animal parasitic nematodes
Animals
Base Sequence
Caenorhabditis briggsae
Caenorhabditis elegans
copy number
DNA Transposable Elements - genetics
DNA, Helminth - analysis
genbank/af099908
Gene Dosage
genetic polymorphism
Genetic Variation
Haemonchus - genetics
Haemonchus contortus
ID Lelystad, Institute for Animal Science and Health
ID-Lelystad, Instituut voor Dierhouderij en Diergezondheid
Inverted repeat
inverted repeats
Molecular Sequence Data
Nematode
nucleotide sequences
Polymorphism, Genetic
repetitive sequences
Repetitive Sequences, Nucleic Acid
Sequence Analysis, DNA
Target site
Tc1
Transposon
transposons
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Summary:Hctc1, a member of the Tc1-family of transposable elements was isolated from the parasitic nematode Haemonchus contortus. Hctc1 is 1590 bp long, is flanked by 55 bp inverted repeats and carries a single open reading frame of a 340 amino acid transposase-like protein. Hctc1 is similar to Tc1 of Caenorhabditis elegans and elements Tcb1 and Tcb2 of Caenorhabditis briggsae in the inverted terminal repeats, the open reading frame, as well as the target insertion sequence. Furthermore, the copy number of Hctc1 is comparable with the Tc1 copy number in low copy strains of C. elegans. The sequence of Hctc1 is highly variable in H. contortus due to deletions, insertions and point mutations, with at least five distinct length variants of Hctc1. Most of the Hctc1 variation was within rather than between H. contortus populations. The high level of sequence variation is probably due to variation generally found for members of the Tc1-family, as well as a high background level of genetic variation of H. contortus.
ISSN:0166-6851
1872-9428
DOI:10.1016/S0166-6851(99)00094-8