Functional Expression on Bacteriophage of the Mustard Trypsin Inhibitor MTI-2

The mustard trypsin inhibitor MTI-2 is a potential tool in the study of interactions between pest insects and plants. It can be applied to study the adaptations of digestive proteases in pest insects. Phage display allows a rapid and exhaustive system for the selection of heterologous protein varian...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-01, Vol.280 (3), p.813-817
Main Authors: Volpicella, Mariateresa, Ceci, Luigi R., Gallerani, Raffaele, Jongsma, Maarten A., Beekwilder, Jules
Format: Article
Language:English
Subjects:
Animals
Base Sequence
DNA Primers - genetics
Escherichia coli - genetics
Gene Expression
Genetic Variation
Mustard Plant - genetics
mustard trypsin inhibitor
Peptide Library
phage display
Pichia - genetics
Pichia pastoris
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Research International
Plants, Medicinal
Protein Engineering
Trypsin Inhibitors - genetics
Trypsin Inhibitors - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The mustard trypsin inhibitor MTI-2 is a potential tool in the study of interactions between pest insects and plants. It can be applied to study the adaptations of digestive proteases in pest insects. Phage display allows a rapid and exhaustive system for the selection of heterologous protein variants with novel specificities. Here we describe a bacteriophage expression system which permits functional expression of MTI-2 variants. Active and inactive mutants of MTI-2 are constructed and displayed on phage. These are used to demonstrate that an active variant can be selected from a background of 10,000 inactive mutants in four rounds of selection and amplification.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.4182