Assessment of metabolic properties and kinetic parameters of methanogenic sludge by on-line methane production rate measurements

This report presents a new approach to studying the metabolic and kinetic properties of anaerobic sludge from single batch experiments. The two main features of the method are that the methane production is measured on-line with a relatively cheap system, and that the methane production data can be...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2002, Vol.58 (2), p.248-254
Main Authors: Gonzalez-Gil, G, Kleerebezem, R, Lettinga, G
Format: Article
Language:English
Subjects:
Anaerobiosis
biodegradability
Biodegradation
Biological and medical sciences
Biological treatment of sewage sludges and wastes
Bioreactors
Biotechnology
case studies
Culture Media
Environment and pollution
Environmental Technology
Euryarchaeota - growth & development
Euryarchaeota - metabolism
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
industry
Kinetics
Laboratorium voor Fysische chemie en Kolloïdkunde
Metabolism
Methane
Methane - metabolism
methane production
methanogens
Milieutechnologie
Models, Biological
Online Systems - instrumentation
Physical Chemistry and Colloid Science
Sectie Milieutechnologie
Sewage - chemistry
Sewage - microbiology
Sludge
Sub-department of Environmental Technology
toxicity testing
WIMEK
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Summary:This report presents a new approach to studying the metabolic and kinetic properties of anaerobic sludge from single batch experiments. The two main features of the method are that the methane production is measured on-line with a relatively cheap system, and that the methane production data can be plotted as rate vs time curves. The case studies of specific methanogenic activity, biodegradability and toxicity tests here presented show that very accurate kinetic data can be obtained. The method is specifically useful in experiments in which strong changes in methane production occur, and it is proposed as a powerful tool to study methanogenic systems. Furthermore, the method is simple and could be implemented by industry in the routine analysis of sludge.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-001-0831-5