Assessment of the microbody luminal pH in the filamentous fungus Penicillium chrysogenum

The enzymes of the penicillin biosynthetic pathway in Penicillium chrysogenum are located in different subcellular compartments. Consequently, penicillin pathway precursors and the biologically active penicillins have to cross one or more membranes. The final enzymatic step that is mediated by acylt...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2002-04, Vol.1589 (2), p.104-111
Main Authors: van der Lende, Ted R, Breeuwer, Pieter, Abee, Tjakko, Konings, Wil N, Driessen, Arnold J.M
Format: Article
Language:English
Subjects:
Fluorescent Dyes
Food Microbiology
Food Microbiology Laboratory
Green Fluorescent Proteins
Hydrogen-Ion Concentration
Laboratorium Levensmiddelenmicrobiologie
Levensmiddelenmicrobiologie
Luminescent Proteins
Microbodies - chemistry
Microbody
Mycelium - chemistry
Organelle
Penicillin
Penicillium chrysogenum
Penicillium chrysogenum - chemistry
Penicillium chrysogenum - ultrastructure
Peroxisome
Spectrometry, Fluorescence
Vacuole
VLAG
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Summary:The enzymes of the penicillin biosynthetic pathway in Penicillium chrysogenum are located in different subcellular compartments. Consequently, penicillin pathway precursors and the biologically active penicillins have to cross one or more membranes. The final enzymatic step that is mediated by acyltransferase takes place in a microbody. The pH of the microbody lumen in penicillin producing cells has been determined with fluorescent probes and mutants of the green fluorescent protein and found to be slightly alkaline.
ISSN:0167-4889
0006-3002
1879-2596
DOI:10.1016/S0167-4889(02)00162-3